Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation
Tuberization in cassava (Manihot esculenta Crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. In potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism an...
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th-mahidol.113152022-09-02T15:18:01Z Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation Mashamon Mitprasat Sittiruk Roytrakul Surasak Jiemsup Opas Boonseng Kittisak Yokthongwattana Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Thailand Ministry of Agriculture and Cooperatives Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Tuberization in cassava (Manihot esculenta Crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. In potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism and regulatory processes in leaf may have an impact on tuber formation. To search for leaf proteins putatively involved in regulating tuber generation and/or development in cassava, comparative proteomic approaches have been applied to monitor differentially expressed leaf proteins during root transition from fibrous to tuberous. Stringent cross comparison and statistical analysis between two groups with different plant ages using Student's t test with 95% significance level revealed a number of protein spots whose abundance were significantly altered (P < 0.05) during week 4 to week 8 of growth. Of these, 39 spots were successfully identified by ion trap LC-MS/MS. The proteins span various functional categories from antioxidant and defense, carbohydrate metabolism, cyanogenesis, energy metabolism, miscellaneous and unknown proteins. Results suggested possible metabolic switches in the leaf that may trigger/regulate storage root initiation and growth. This study provides a basis for further functional characterization of differentially expressed leaf proteins, which can help understand how biochemical processes in cassava leaves may be involved in storage root development. © 2011 Springer-Verlag. 2018-05-03T07:56:33Z 2018-05-03T07:56:33Z 2011-06-01 Article Planta. Vol.233, No.6 (2011), 1209-1221 10.1007/s00425-011-1373-4 14322048 00320935 2-s2.0-79957624792 https://repository.li.mahidol.ac.th/handle/123456789/11315 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79957624792&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Mashamon Mitprasat Sittiruk Roytrakul Surasak Jiemsup Opas Boonseng Kittisak Yokthongwattana Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation |
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Tuberization in cassava (Manihot esculenta Crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. In potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism and regulatory processes in leaf may have an impact on tuber formation. To search for leaf proteins putatively involved in regulating tuber generation and/or development in cassava, comparative proteomic approaches have been applied to monitor differentially expressed leaf proteins during root transition from fibrous to tuberous. Stringent cross comparison and statistical analysis between two groups with different plant ages using Student's t test with 95% significance level revealed a number of protein spots whose abundance were significantly altered (P < 0.05) during week 4 to week 8 of growth. Of these, 39 spots were successfully identified by ion trap LC-MS/MS. The proteins span various functional categories from antioxidant and defense, carbohydrate metabolism, cyanogenesis, energy metabolism, miscellaneous and unknown proteins. Results suggested possible metabolic switches in the leaf that may trigger/regulate storage root initiation and growth. This study provides a basis for further functional characterization of differentially expressed leaf proteins, which can help understand how biochemical processes in cassava leaves may be involved in storage root development. © 2011 Springer-Verlag. |
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Mahidol University Mashamon Mitprasat Sittiruk Roytrakul Surasak Jiemsup Opas Boonseng Kittisak Yokthongwattana |
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Mashamon Mitprasat Sittiruk Roytrakul Surasak Jiemsup Opas Boonseng Kittisak Yokthongwattana |
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Mashamon Mitprasat |
title |
Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation |
title_short |
Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation |
title_full |
Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation |
title_fullStr |
Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation |
title_full_unstemmed |
Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation |
title_sort |
leaf proteomic analysis in cassava (manihot esculenta, crantz) during plant development, from planting of stem cutting to storage root formation |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/11315 |
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1763493976630886400 |