Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment

Artemisinin is a promising and potent antimalarial drug naturally produced by the plant Artemisia annua L. but in very low yield. Its artemisinin content is known to be greatly affected by both genotype and environmental factors. In this study, the production of artemisinin and leaf biomass in Artem...

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Main Authors: Waleerat Banyai, Masahiro Mii, Kanyaratt Supaibulwatana
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/11373
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spelling th-mahidol.113732018-05-03T15:04:33Z Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment Waleerat Banyai Masahiro Mii Kanyaratt Supaibulwatana Mahidol University Chiba University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Artemisinin is a promising and potent antimalarial drug naturally produced by the plant Artemisia annua L. but in very low yield. Its artemisinin content is known to be greatly affected by both genotype and environmental factors. In this study, the production of artemisinin and leaf biomass in Artemisia annua L. was significantly increased by exogenous GA 3 treatment. The effect of GA 3 application on expression of proposed key enzymes involved in artemisinin yield was examined in both wild type (007) and FPS-overexpression (253-2) lines of A. annua. In the wild type (007) at 6 h post GA 3 application there was an abrupt rise in FPS, ADS and CYP71AV1 expression and at 24 h a temporary and significant peak in artemisinin (1.45-fold higher than the control). After GA 3 application in line 253-2, there was a dramatic rise in expression of FPS at 3 h, CYP71AV1 at 9 h and ADS at 72 h and accumulation of artemisinin after 7 days, which was a delay when compared with the wild type plant. Thus, increased artemisinin content from exogenous GA 3 treatment was associated with increased expression of key enzymes in the artemisinin biosynthesis pathway. Interestingly, exogenous GA 3 continuously enhanced artemisinin content from the vegetative stage to flower initiation in both plant lines and gave significantly higher leaf biomass than in control plants. Consequently, the artemisinin yield in GA 3 -treated plants was much higher than in control plants. Although the maximum artemisinin content was found at the full blooming stage [2.1% dry weight (DW) in 007 and 2.4% DW in 253-2], the highest artemisinin yield in GA 3 -treated plants was obtained during the flower initiation stage (2.4 mg/plant in 007 and 2.3 mg/plant in 235-2). This was 26.3 and 27.8% higher, respectively, than in non-treated plants 007 and 253-2. This study showed that exogenous GA 3 treatment enhanced artemisinin production in pot experiments and should be suitable for field application. © 2010 Springer Science+Business Media B.V. 2018-05-03T07:57:48Z 2018-05-03T07:57:48Z 2011-01-01 Article Plant Growth Regulation. Vol.63, No.1 (2011), 45-54 10.1007/s10725-010-9510-9 15735087 01676903 2-s2.0-78650305586 https://repository.li.mahidol.ac.th/handle/123456789/11373 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78650305586&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Waleerat Banyai
Masahiro Mii
Kanyaratt Supaibulwatana
Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment
description Artemisinin is a promising and potent antimalarial drug naturally produced by the plant Artemisia annua L. but in very low yield. Its artemisinin content is known to be greatly affected by both genotype and environmental factors. In this study, the production of artemisinin and leaf biomass in Artemisia annua L. was significantly increased by exogenous GA 3 treatment. The effect of GA 3 application on expression of proposed key enzymes involved in artemisinin yield was examined in both wild type (007) and FPS-overexpression (253-2) lines of A. annua. In the wild type (007) at 6 h post GA 3 application there was an abrupt rise in FPS, ADS and CYP71AV1 expression and at 24 h a temporary and significant peak in artemisinin (1.45-fold higher than the control). After GA 3 application in line 253-2, there was a dramatic rise in expression of FPS at 3 h, CYP71AV1 at 9 h and ADS at 72 h and accumulation of artemisinin after 7 days, which was a delay when compared with the wild type plant. Thus, increased artemisinin content from exogenous GA 3 treatment was associated with increased expression of key enzymes in the artemisinin biosynthesis pathway. Interestingly, exogenous GA 3 continuously enhanced artemisinin content from the vegetative stage to flower initiation in both plant lines and gave significantly higher leaf biomass than in control plants. Consequently, the artemisinin yield in GA 3 -treated plants was much higher than in control plants. Although the maximum artemisinin content was found at the full blooming stage [2.1% dry weight (DW) in 007 and 2.4% DW in 253-2], the highest artemisinin yield in GA 3 -treated plants was obtained during the flower initiation stage (2.4 mg/plant in 007 and 2.3 mg/plant in 235-2). This was 26.3 and 27.8% higher, respectively, than in non-treated plants 007 and 253-2. This study showed that exogenous GA 3 treatment enhanced artemisinin production in pot experiments and should be suitable for field application. © 2010 Springer Science+Business Media B.V.
author2 Mahidol University
author_facet Mahidol University
Waleerat Banyai
Masahiro Mii
Kanyaratt Supaibulwatana
format Article
author Waleerat Banyai
Masahiro Mii
Kanyaratt Supaibulwatana
author_sort Waleerat Banyai
title Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment
title_short Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment
title_full Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment
title_fullStr Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment
title_full_unstemmed Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA<inf>3</inf>treatment
title_sort enhancement of artemisinin content and biomass in artemisia annua by exogenous ga<inf>3</inf>treatment
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/11373
_version_ 1763496023740645376