The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule
The stress-responsive alternative sigma factor σ B is conserved across diverse Gram-positive bacterial genera. In Listeria monocytogenes, σ B regulates transcription of > 150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such...
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th-mahidol.119672018-05-03T15:14:15Z The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule M. Elizabeth Palmer Soraya Chaturongakul Martin Wiedmann Kathryn J. Boor Cornell University Mahidol University Immunology and Microbiology The stress-responsive alternative sigma factor σ B is conserved across diverse Gram-positive bacterial genera. In Listeria monocytogenes, σ B regulates transcription of > 150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such as those encountered in the human gastrointestinal lumen. An inhibitor of L. monocytogenes σ B activity was identified by screening ~57,000 natural and synthesized small molecules using a high-throughput cell-based assay. The compound fluoro-phenyl-styrene-sulfonamide (FPSS) (IC 50 = 3.5 μM) downregulated the majority of genes previously identified as members of the σ B regulon in L. monocytogenes 10403S, thus generating a transcriptional profile comparable to that of a 10403S ΔsigB strain. Specifically, of the 208 genes downregulated by FPSS, 75% had been identified previously as positively regulated by σ B . Downregulated genes included key virulence and stress response genes, such as inlA, inlB, bsh, hfq, opuC, and bilE. From a functional perspective, FPSS also inhibited L. monocytogenes invasion of human intestinal epithelial cells and bile salt hydrolase activity. The ability of FPSS to inhibit σ B activity in both L. monocytogenes and Bacillus subtilis indicates its utility as a specific inhibitor of σ B across multiple Gram-positive genera. © 2011 Palmer et al. 2018-05-03T08:14:15Z 2018-05-03T08:14:15Z 2011-11-01 Article mBio. Vol.2, No.6 (2011) 10.1128/mBio.00241-11 21507511 2-s2.0-84855212172 https://repository.li.mahidol.ac.th/handle/123456789/11967 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84855212172&origin=inward |
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Immunology and Microbiology M. Elizabeth Palmer Soraya Chaturongakul Martin Wiedmann Kathryn J. Boor The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
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The stress-responsive alternative sigma factor σ B is conserved across diverse Gram-positive bacterial genera. In Listeria monocytogenes, σ B regulates transcription of > 150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such as those encountered in the human gastrointestinal lumen. An inhibitor of L. monocytogenes σ B activity was identified by screening ~57,000 natural and synthesized small molecules using a high-throughput cell-based assay. The compound fluoro-phenyl-styrene-sulfonamide (FPSS) (IC 50 = 3.5 μM) downregulated the majority of genes previously identified as members of the σ B regulon in L. monocytogenes 10403S, thus generating a transcriptional profile comparable to that of a 10403S ΔsigB strain. Specifically, of the 208 genes downregulated by FPSS, 75% had been identified previously as positively regulated by σ B . Downregulated genes included key virulence and stress response genes, such as inlA, inlB, bsh, hfq, opuC, and bilE. From a functional perspective, FPSS also inhibited L. monocytogenes invasion of human intestinal epithelial cells and bile salt hydrolase activity. The ability of FPSS to inhibit σ B activity in both L. monocytogenes and Bacillus subtilis indicates its utility as a specific inhibitor of σ B across multiple Gram-positive genera. © 2011 Palmer et al. |
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Cornell University |
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Cornell University M. Elizabeth Palmer Soraya Chaturongakul Martin Wiedmann Kathryn J. Boor |
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M. Elizabeth Palmer Soraya Chaturongakul Martin Wiedmann Kathryn J. Boor |
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M. Elizabeth Palmer |
title |
The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
title_short |
The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
title_full |
The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
title_fullStr |
The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
title_full_unstemmed |
The Listeria monocytogenes σ<sup>B</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
title_sort |
listeria monocytogenes σ<sup>b</sup>regulon and its virulence-associated functions are inhibited by a small molecule |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/11967 |
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1763498132739457024 |