Detection of infectious myonecrosis virus using monoclonal antibody specific to N and C fragments of the capsid protein expressed heterologously

• Main Authors: Areerat Kunanopparat, Parin Chaivisuthangkura, Saengchan Senapin, Siwaporn Longyant, Sombat Rukpratanporn, Timothy W. Flegel, Paisarn Sithigorngul
• Other Authors: Srinakharinwirot University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/12091
• Institution: Mahidol University

The gene encoding the capsid protein in ORF1 of the genome of infectious myonecrosis virus (IMNV) (GenBank AY570982) was amplified into three parts named CP-N (nucleotides 2248-3045), CP-I (nucleotides 3046-3954) and CP-C (nucleotides 3955-4953). The CP-N fragment was inserted into expression vector...