Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection

• Main Authors: Damodar Paudel, Richard Jarman, Kriengsak Limkittikul, Chonticha Klungthong, Supat Chamnanchanunt, Ananda Nisalak, Robert Gibbons, Watcharee Chokejindachai
• Other Authors: Nepal Orthopaedic Hospital
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/12293
• Institution: Mahidol University

Background: Dengue fever and dengue hemorrhagic fever are caused by dengue virus. Dengue infection remains a burning problem of many countries. To diagnose acute dengue in the early phase we improve the low cost, rapid SYBR green real time assay and compared the sensitivity and specificity with real...