Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis
Background. We aimed to determine the antibody and T cell responses to Burkholderia pseudomallei of humans to select candidate vaccine antigens. Methods. For antibody profiling, a protein microarray of 154 B. pseudomallei proteins was probed with plasma from 108 healthy individuals and 72 recovered...
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th-mahidol.125772018-05-03T15:33:51Z Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis Duangchan Suwannasaen Jirawan Mahawantung Wipada Chaowagul Direk Limmathurotsakul Philip L. Felgner Huw Davies Gregory J. Bancroft Richard W. Titball Ganjana Lertmemongkolchai Khon Kaen University Sappasitthiprasong Hospital Mahidol University University of California, Irvine University of Exeter Medicine Background. We aimed to determine the antibody and T cell responses to Burkholderia pseudomallei of humans to select candidate vaccine antigens. Methods. For antibody profiling, a protein microarray of 154 B. pseudomallei proteins was probed with plasma from 108 healthy individuals and 72 recovered patients. Blood from 20 of the healthy and 30 of the recovered individuals was also obtained for T cell assays. Results. Twenty-seven proteins distinctively reacted with human plasma following environmental exposure or clinical melioidosis. We compared the responses according to the patient's history of subsequent relapse, and antibody response to BPSL2765 was higher in plasma from individuals who had only 1 episode of disease than in those with recurrent melioidosis. A comparison of antibody and T cell responses to 5 B. pseudomallei proteins revealed that BimA and flagellin-induced responses were similar but that BPSS0530 could induce T cell responses in healthy controls more than in recovered patients. Conclusions. By combining large-scale antibody microarrays and assays of T cell-mediated immunity, we identified a panel of novel B. pseudomallei proteins that show distinct patterns of reactivity in different stages of human melioidosis. These proteins may be useful candidates for development of subunit-based vaccines and in monitoring the risks of treatment failure and relapse. © The Author 2011. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. 2018-05-03T08:33:51Z 2018-05-03T08:33:51Z 2011-04-01 Article Journal of Infectious Diseases. Vol.203, No.7 (2011), 1002-1011 10.1093/infdis/jiq142 00221899 2-s2.0-79953019437 https://repository.li.mahidol.ac.th/handle/123456789/12577 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79953019437&origin=inward |
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Medicine Duangchan Suwannasaen Jirawan Mahawantung Wipada Chaowagul Direk Limmathurotsakul Philip L. Felgner Huw Davies Gregory J. Bancroft Richard W. Titball Ganjana Lertmemongkolchai Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis |
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Background. We aimed to determine the antibody and T cell responses to Burkholderia pseudomallei of humans to select candidate vaccine antigens. Methods. For antibody profiling, a protein microarray of 154 B. pseudomallei proteins was probed with plasma from 108 healthy individuals and 72 recovered patients. Blood from 20 of the healthy and 30 of the recovered individuals was also obtained for T cell assays. Results. Twenty-seven proteins distinctively reacted with human plasma following environmental exposure or clinical melioidosis. We compared the responses according to the patient's history of subsequent relapse, and antibody response to BPSL2765 was higher in plasma from individuals who had only 1 episode of disease than in those with recurrent melioidosis. A comparison of antibody and T cell responses to 5 B. pseudomallei proteins revealed that BimA and flagellin-induced responses were similar but that BPSS0530 could induce T cell responses in healthy controls more than in recovered patients. Conclusions. By combining large-scale antibody microarrays and assays of T cell-mediated immunity, we identified a panel of novel B. pseudomallei proteins that show distinct patterns of reactivity in different stages of human melioidosis. These proteins may be useful candidates for development of subunit-based vaccines and in monitoring the risks of treatment failure and relapse. © The Author 2011. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. |
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Khon Kaen University |
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Khon Kaen University Duangchan Suwannasaen Jirawan Mahawantung Wipada Chaowagul Direk Limmathurotsakul Philip L. Felgner Huw Davies Gregory J. Bancroft Richard W. Titball Ganjana Lertmemongkolchai |
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Article |
author |
Duangchan Suwannasaen Jirawan Mahawantung Wipada Chaowagul Direk Limmathurotsakul Philip L. Felgner Huw Davies Gregory J. Bancroft Richard W. Titball Ganjana Lertmemongkolchai |
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Duangchan Suwannasaen |
title |
Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis |
title_short |
Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis |
title_full |
Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis |
title_fullStr |
Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis |
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Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis |
title_sort |
human immune responses to burkholderia pseudomallei characterized by protein microarray analysis |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/12577 |
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1763488934744031232 |