Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent
By use of the neutral alkylating agent iodo [ 14 C 1 ] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including t...
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th-mahidol.132472018-06-01T13:31:07Z Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent Jisnuson Svasti Napasri Talupphet Mahidol University Medicine By use of the neutral alkylating agent iodo [ 14 C 1 ] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis. © 1979. 2018-06-01T06:31:07Z 2018-06-01T06:31:07Z 1979-03-27 Article BBA - Protein Structure. Vol.577, No.1 (1979), 221-225 10.1016/0005-2795(79)90024-2 00052795 2-s2.0-0018802443 https://repository.li.mahidol.ac.th/handle/123456789/13247 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0018802443&origin=inward |
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Medicine Jisnuson Svasti Napasri Talupphet Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| description |
By use of the neutral alkylating agent iodo [ 14 C 1 ] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis. © 1979. |
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Mahidol University |
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Mahidol University Jisnuson Svasti Napasri Talupphet |
| format |
Article |
| author |
Jisnuson Svasti Napasri Talupphet |
| author_sort |
Jisnuson Svasti |
| title |
Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| title_short |
Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| title_full |
Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| title_fullStr |
Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| title_full_unstemmed |
Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| title_sort |
improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent |
| publishDate |
2018 |
| url |
https://repository.li.mahidol.ac.th/handle/123456789/13247 |
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1763489195440996352 |