High confidence prediction of essential genes in Burkholderia Cenocepacia
Background: Essential genes are absolutely required for the survival of an organism. The identification of essential genes, besides being one of the most fundamental questions in biology, is also of interest for the emerging science of synthetic biology and for the development of novel antimicrobial...
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th-mahidol.134452018-06-11T12:09:14Z High confidence prediction of essential genes in Burkholderia Cenocepacia Mario Juhas Manuel Stark Christian von Mering Puthapoom Lumjiaktase Derrick W. Crook Miguel A. Valvano Leo Eberl Universitat Zurich Mahidol University Nuffield Department of Clinical Medicine Western University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Medicine Background: Essential genes are absolutely required for the survival of an organism. The identification of essential genes, besides being one of the most fundamental questions in biology, is also of interest for the emerging science of synthetic biology and for the development of novel antimicrobials. New antimicrobial therapies are desperately needed to treat multidrug-resistant pathogens, such as members of the Burkholderia cepacia complex. Methodology/Principal Findings: We hypothesize that essential genes may be highly conserved within a group of evolutionary closely related organisms. Using a bioinformatics approach we determined that the core genome of the order Burkholderiales consists of 649 genes. All but two of these identified genes were located on chromosome 1 of Burkholderia cenocepacia. Although many of the 649 core genes of Burkholderiales have been shown to be essential in other bacteria, we were also able to identify a number of novel essential genes present mainly, or exclusively, within this order. The essentiality of some of the core genes, including the known essential genes infB, gyrB, ubiB, and valS, as well as the so far uncharacterized genes BCAL1882, BCAL2769, BCAL3142 and BCAL3369 has been confirmed experimentally in B. cenocepacia. Conclusions/Significance: We report on the identification of essential genes using a novel bioinformatics strategy and provide bioinformatics and experimental evidence that the large majority of the identified genes are indeed essential. The essential genes identified here may represent valuable targets for the development of novel antimicrobials and their detailed study may shed new light on the functions required to support life. © 2012 Juhas et al. 2018-06-11T04:30:31Z 2018-06-11T04:30:31Z 2012-06-29 Article PLoS ONE. Vol.7, No.6 (2012) 10.1371/journal.pone.0040064 19326203 2-s2.0-84863104679 https://repository.li.mahidol.ac.th/handle/123456789/13445 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84863104679&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Medicine Mario Juhas Manuel Stark Christian von Mering Puthapoom Lumjiaktase Derrick W. Crook Miguel A. Valvano Leo Eberl High confidence prediction of essential genes in Burkholderia Cenocepacia |
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Background: Essential genes are absolutely required for the survival of an organism. The identification of essential genes, besides being one of the most fundamental questions in biology, is also of interest for the emerging science of synthetic biology and for the development of novel antimicrobials. New antimicrobial therapies are desperately needed to treat multidrug-resistant pathogens, such as members of the Burkholderia cepacia complex. Methodology/Principal Findings: We hypothesize that essential genes may be highly conserved within a group of evolutionary closely related organisms. Using a bioinformatics approach we determined that the core genome of the order Burkholderiales consists of 649 genes. All but two of these identified genes were located on chromosome 1 of Burkholderia cenocepacia. Although many of the 649 core genes of Burkholderiales have been shown to be essential in other bacteria, we were also able to identify a number of novel essential genes present mainly, or exclusively, within this order. The essentiality of some of the core genes, including the known essential genes infB, gyrB, ubiB, and valS, as well as the so far uncharacterized genes BCAL1882, BCAL2769, BCAL3142 and BCAL3369 has been confirmed experimentally in B. cenocepacia. Conclusions/Significance: We report on the identification of essential genes using a novel bioinformatics strategy and provide bioinformatics and experimental evidence that the large majority of the identified genes are indeed essential. The essential genes identified here may represent valuable targets for the development of novel antimicrobials and their detailed study may shed new light on the functions required to support life. © 2012 Juhas et al. |
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Universitat Zurich |
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Universitat Zurich Mario Juhas Manuel Stark Christian von Mering Puthapoom Lumjiaktase Derrick W. Crook Miguel A. Valvano Leo Eberl |
format |
Article |
author |
Mario Juhas Manuel Stark Christian von Mering Puthapoom Lumjiaktase Derrick W. Crook Miguel A. Valvano Leo Eberl |
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Mario Juhas |
title |
High confidence prediction of essential genes in Burkholderia Cenocepacia |
title_short |
High confidence prediction of essential genes in Burkholderia Cenocepacia |
title_full |
High confidence prediction of essential genes in Burkholderia Cenocepacia |
title_fullStr |
High confidence prediction of essential genes in Burkholderia Cenocepacia |
title_full_unstemmed |
High confidence prediction of essential genes in Burkholderia Cenocepacia |
title_sort |
high confidence prediction of essential genes in burkholderia cenocepacia |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/13445 |
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1763496162040479744 |