Development and validation of burkholderia pseudomallei-specific real-time pcr assays for clinical, environmental or forensic detection applications

• Main Authors: Erin P. Price, Julia L. Dale, James M. Cook, Derek S. Sarovich, Meagan L. Seymour, Jennifer L. Ginther, Emily L. Kaufman, Stephen M. Beckstrom-Sternberg, Mark Mayo, Mirjam Kaestli, Mindy B. Glass, Jay E. Gee, Vanaporn Wuthiekanun, Jeffrey M. Warner, Anthony Baker, Jeffrey T. Foster, Patrick Tan, Apichai Tuanyok, Direk Limmathurotsakul, Sharon J. Peacock, Bart J. Currie, David M. Wagner, Paul Keim, Talima Pearson
• Other Authors: Northern Arizona University
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13454
• Institution: Mahidol University

The bacterium Burkholderia pseudomallei causes melioidosis, a rare but serious illness that can be fatal if untreated or misdiagnosed. Species-specific PCR assays provide a technically simple method for differentiating B. pseudomallei from near-neighbor species. However, substantial genetic diversit...