Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil

• Main Authors: Direk Limmathurotsakul, Vanaporn Wuthiekanun, Premjit Amornchai, Gumphol Wongsuwan, Nicholas P J Day, Sharon J. Peacock
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Environmental Science; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13503

Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to t...