Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats
Insulin resistance of skeletal muscle glucose transport due to prolonged loss of ovarian function in ovariectomized (OVX) rats is accompanied by other features of the metabolic syndrome and may be confounded by increased calorie consumption. In this study, we investigated the role of calorie consump...
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th-mahidol.137022018-06-11T11:36:19Z Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats Mujalin Prasannarong Kanokwan Vichaiwong Vitoon Saengsirisuwan Mahidol University Biochemistry, Genetics and Molecular Biology Insulin resistance of skeletal muscle glucose transport due to prolonged loss of ovarian function in ovariectomized (OVX) rats is accompanied by other features of the metabolic syndrome and may be confounded by increased calorie consumption. In this study, we investigated the role of calorie consumption in the development of insulin resistance in OVX rats. In addition, we examined the cellular mechanisms underlying skeletal muscle insulin resistance in OVX rats. Female Sprague-Dawley rats were ovariectomized (OVX) or sham-operated (SHAM). OVX rats either had free access to food, pair feeding (PF) with SHAM or received a 35% reduction in food intake (calorie restriction; CR) for 12weeks. Compared with SHAM, ovariectomy induced skeletal muscle insulin resistance, which was associated with decreases (32-70%) in tyrosine phosphorylation of the insulin receptor and insulin receptor substrate-1 (IRS-1), IRS-1 associated p85 subunit of phosphatidylinositol 3-kinase (PI3-kinase), and Akt Ser 473 phosphorylation whereas insulin-stimulated phosphorylation of IRS-1 Ser 307 , SAPK/JNK Thr 183 /Tyr 185 , and p38 mitogen-activated protein kinase (MAPK) Thr 180 /Tyr 182 was increased (24-62%). PF improved the serum lipid profile but did not restore insulin-stimulated glucose transport, indicating that insulin resistance in OVX rats is a conseque nce of ovarian hormone deprivation. In contrast, impaired insulin sensitivity and defective insulin signaling were not observed in the skeletal muscle of OVX+CR rats. Therefore, we provide evidence for the first time that CR effectively prevents the development of insulin resistance and impaired insulin signaling in the skeletal muscle of OVX rats. © 2012 Elsevier B.V. 2018-06-11T04:36:19Z 2018-06-11T04:36:19Z 2012-06-01 Article Biochimica et Biophysica Acta - Molecular Basis of Disease. Vol.1822, No.6 (2012), 1051-1061 10.1016/j.bbadis.2012.02.018 1879260X 09254439 2-s2.0-84859056344 https://repository.li.mahidol.ac.th/handle/123456789/13702 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859056344&origin=inward |
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Biochemistry, Genetics and Molecular Biology Mujalin Prasannarong Kanokwan Vichaiwong Vitoon Saengsirisuwan Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
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Insulin resistance of skeletal muscle glucose transport due to prolonged loss of ovarian function in ovariectomized (OVX) rats is accompanied by other features of the metabolic syndrome and may be confounded by increased calorie consumption. In this study, we investigated the role of calorie consumption in the development of insulin resistance in OVX rats. In addition, we examined the cellular mechanisms underlying skeletal muscle insulin resistance in OVX rats. Female Sprague-Dawley rats were ovariectomized (OVX) or sham-operated (SHAM). OVX rats either had free access to food, pair feeding (PF) with SHAM or received a 35% reduction in food intake (calorie restriction; CR) for 12weeks. Compared with SHAM, ovariectomy induced skeletal muscle insulin resistance, which was associated with decreases (32-70%) in tyrosine phosphorylation of the insulin receptor and insulin receptor substrate-1 (IRS-1), IRS-1 associated p85 subunit of phosphatidylinositol 3-kinase (PI3-kinase), and Akt Ser 473 phosphorylation whereas insulin-stimulated phosphorylation of IRS-1 Ser 307 , SAPK/JNK Thr 183 /Tyr 185 , and p38 mitogen-activated protein kinase (MAPK) Thr 180 /Tyr 182 was increased (24-62%). PF improved the serum lipid profile but did not restore insulin-stimulated glucose transport, indicating that insulin resistance in OVX rats is a conseque nce of ovarian hormone deprivation. In contrast, impaired insulin sensitivity and defective insulin signaling were not observed in the skeletal muscle of OVX+CR rats. Therefore, we provide evidence for the first time that CR effectively prevents the development of insulin resistance and impaired insulin signaling in the skeletal muscle of OVX rats. © 2012 Elsevier B.V. |
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Mahidol University |
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Mahidol University Mujalin Prasannarong Kanokwan Vichaiwong Vitoon Saengsirisuwan |
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Article |
author |
Mujalin Prasannarong Kanokwan Vichaiwong Vitoon Saengsirisuwan |
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Mujalin Prasannarong |
title |
Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
title_short |
Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
title_full |
Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
title_fullStr |
Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
title_full_unstemmed |
Calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
title_sort |
calorie restriction prevents the development of insulin resistance and impaired insulin signaling in skeletal muscle of ovariectomized rats |
publishDate |
2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/13702 |
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1763492286806622208 |