Purification and characterization of phytase from Klebsiella pneumoniae 9-3B

• Main Authors: Lotis Escobin-Mopera, Midori Ohtani, Sachie Sekiguchi, Teruo Sone, Ayumi Abe, Michiko Tanaka, Vithaya Meevootisom, Kozo Asano
• Other Authors: Hokkaido University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13745
• Institution: Mahidol University

Phytase, an enzyme that catalyzes the hydrolysis of phytate, was purified from Klebsiella pneumoniae 9-3B. The isolate was preferentially selected in a medium which contains phytate as a sole carbon and phosphate source. Phytic acid was utilized for growth and consequently stimulated phytase product...