Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol

Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridizat...

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Main Authors: Suthee Benjaphokee, Daisuke Hasegawa, Daiki Yokota, Thipa Asvarak, Choowong Auesukaree, Minetaka Sugiyama, Yoshinobu Kaneko, Chuenchit Boonchird, Satoshi Harashima
Other Authors: Osaka University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/13800
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spelling th-mahidol.138002018-06-11T11:41:56Z Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol Suthee Benjaphokee Daisuke Hasegawa Daiki Yokota Thipa Asvarak Choowong Auesukaree Minetaka Sugiyama Yoshinobu Kaneko Chuenchit Boonchird Satoshi Harashima Osaka University Mahidol University Biochemistry, Genetics and Molecular Biology Chemical Engineering Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridization technique without recombinant DNA technology. A heterothallic strain showing a high-temperature (41°C) tolerant (Htg + ) phenotype, a derivative from a strain isolated from nature, was crossed with a homothallic strain displaying high-ethanol productivity (Hep + ), a stock culture at the Thailand Institute of Scientific and Technological Research. The resu ltant hybrid TJ14 displayed ability to rapidly utilize glucose, and produced ethanol (46.6g/l) from 10% glucose fermentation medium at high temperature (41°C). Not only ethanol productivity at 41°C but also acid tolerance (Acd + ) was improved in TJ14 as compared with its parental strains, enabling TJ14 to grow in liquid medium even at pH 3. TJ14 maintained high ethanol productivity (46.0g/l) from 10% glucose when fermentation was done under multiple-stress conditions (41°C and pH 3.5). Furthermore, when TJ14 was subjected to a repeated-batch fermentation scheme, the growth and ethanol production of TJ14 were maintained at excellent levels over ten cycles of fermentation. Thus, the multiple-stress (Htg + Hep + Acd + ) resistant strain TJ14 should be useful for cost-effective bioethanol production under high-temperature and acidic conditions. © 2011 Elsevier B.V. 2018-06-11T04:39:14Z 2018-06-11T04:39:14Z 2012-02-15 Article New Biotechnology. Vol.29, No.3 (2012), 379-386 10.1016/j.nbt.2011.07.002 18764347 18716784 2-s2.0-84856402141 https://repository.li.mahidol.ac.th/handle/123456789/13800 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856402141&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Suthee Benjaphokee
Daisuke Hasegawa
Daiki Yokota
Thipa Asvarak
Choowong Auesukaree
Minetaka Sugiyama
Yoshinobu Kaneko
Chuenchit Boonchird
Satoshi Harashima
Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
description Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridization technique without recombinant DNA technology. A heterothallic strain showing a high-temperature (41°C) tolerant (Htg + ) phenotype, a derivative from a strain isolated from nature, was crossed with a homothallic strain displaying high-ethanol productivity (Hep + ), a stock culture at the Thailand Institute of Scientific and Technological Research. The resu ltant hybrid TJ14 displayed ability to rapidly utilize glucose, and produced ethanol (46.6g/l) from 10% glucose fermentation medium at high temperature (41°C). Not only ethanol productivity at 41°C but also acid tolerance (Acd + ) was improved in TJ14 as compared with its parental strains, enabling TJ14 to grow in liquid medium even at pH 3. TJ14 maintained high ethanol productivity (46.0g/l) from 10% glucose when fermentation was done under multiple-stress conditions (41°C and pH 3.5). Furthermore, when TJ14 was subjected to a repeated-batch fermentation scheme, the growth and ethanol production of TJ14 were maintained at excellent levels over ten cycles of fermentation. Thus, the multiple-stress (Htg + Hep + Acd + ) resistant strain TJ14 should be useful for cost-effective bioethanol production under high-temperature and acidic conditions. © 2011 Elsevier B.V.
author2 Osaka University
author_facet Osaka University
Suthee Benjaphokee
Daisuke Hasegawa
Daiki Yokota
Thipa Asvarak
Choowong Auesukaree
Minetaka Sugiyama
Yoshinobu Kaneko
Chuenchit Boonchird
Satoshi Harashima
format Article
author Suthee Benjaphokee
Daisuke Hasegawa
Daiki Yokota
Thipa Asvarak
Choowong Auesukaree
Minetaka Sugiyama
Yoshinobu Kaneko
Chuenchit Boonchird
Satoshi Harashima
author_sort Suthee Benjaphokee
title Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
title_short Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
title_full Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
title_fullStr Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
title_full_unstemmed Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
title_sort highly efficient bioethanol production by a saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/13800
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