Development of immortalized hepatocyte-like cells from hMSCs
Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferat...
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th-mahidol.138212018-06-11T11:39:44Z Development of immortalized hepatocyte-like cells from hMSCs Adisak Wongkajornsilp Khanit Sa-Ngiamsuntorn Suradej Hongeng Mahidol University Biochemistry, Genetics and Molecular Biology Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10-20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies. © 2012 Springer Science+Business Media, LLC. 2018-06-11T04:39:44Z 2018-06-11T04:39:44Z 2012-01-20 Article Methods in Molecular Biology. Vol.826, (2012), 73-87 10.1007/978-1-61779-468-1_7 10643745 2-s2.0-84855910106 https://repository.li.mahidol.ac.th/handle/123456789/13821 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84855910106&origin=inward |
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Biochemistry, Genetics and Molecular Biology Adisak Wongkajornsilp Khanit Sa-Ngiamsuntorn Suradej Hongeng Development of immortalized hepatocyte-like cells from hMSCs |
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Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10-20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies. © 2012 Springer Science+Business Media, LLC. |
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Mahidol University |
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Mahidol University Adisak Wongkajornsilp Khanit Sa-Ngiamsuntorn Suradej Hongeng |
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Article |
author |
Adisak Wongkajornsilp Khanit Sa-Ngiamsuntorn Suradej Hongeng |
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Adisak Wongkajornsilp |
title |
Development of immortalized hepatocyte-like cells from hMSCs |
title_short |
Development of immortalized hepatocyte-like cells from hMSCs |
title_full |
Development of immortalized hepatocyte-like cells from hMSCs |
title_fullStr |
Development of immortalized hepatocyte-like cells from hMSCs |
title_full_unstemmed |
Development of immortalized hepatocyte-like cells from hMSCs |
title_sort |
development of immortalized hepatocyte-like cells from hmscs |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/13821 |
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1763491278059732992 |