Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor

• Main Authors: Ekkachai Kittiloespaisan, Akio Ojida, Itaru Hamachi, Yortyot Seetang-Nun, Wansika Kiatpathomchai, Jirarut Wongkongkatep
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Chemistry
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13932

A label-free fluorescent detection method for the loopmediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthenebased Zn(II)-coordination chemosensor 12Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-produ...