On-chip irreversible electroporation for bacterial cell membrane rupture
In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration...
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| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/14128 |
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| Institution: | Mahidol University |
| Summary: | In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration in microsecond. Fluorescent spectrophotometer was employed for live/dead bacterial cells detection. The live bacterial cells were tagged by SYTO 9 green fluorescent stain. The red-fluorescent nucleic acid stain, propidium iodide was used to tag the dead cells. The highest percentage of 95% dead cells was at 7V applied voltage and 90 μs pulse duration. This result was confirmed by plate count with completely dead cell. ©2012 IEEE. |
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