Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture
The establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been l...
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th-mahidol.143592018-06-11T12:17:23Z Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture Céline Borlon Bruce Russell Kanlaya Sriprawat Rossarin Suwanarusk Annette Erhart Laurent Renia François Nosten Umberto D'Alessandro Prins Leopold Instituut voor Tropische Geneeskunde Agency for Science, Technology and Research, Singapore Shoklo Malaria Research Unit Mahidol University Nuffield Department of Clinical Medicine Medical Research Council (MRC) Immunology and Microbiology Medicine The establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been limited by the need for fresh parasite isolates. We explored the possibility of using parasite isolates and reticulocytes, both cryopreserved, to perform invasion and initiate short-term culture. Invasion results obtained with both cryopreserved isolates and reticulocytes were similar to those obtained with fresh samples. This method should be easily replicated in laboratories outside endemic areas and will substantially contribute to the development of a continuous P. vivax culture. In addition, this model could be used for testing vaccine candidates as well as for studying invasion-specific molecular mechanisms. © 2012 Australian Society for Parasitology Inc. 2018-06-11T04:54:59Z 2018-06-11T04:54:59Z 2012-02-01 Article International Journal for Parasitology. Vol.42, No.2 (2012), 155-160 10.1016/j.ijpara.2011.10.011 18790135 00207519 2-s2.0-84856506075 https://repository.li.mahidol.ac.th/handle/123456789/14359 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856506075&origin=inward |
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Immunology and Microbiology Medicine Céline Borlon Bruce Russell Kanlaya Sriprawat Rossarin Suwanarusk Annette Erhart Laurent Renia François Nosten Umberto D'Alessandro Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
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The establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been limited by the need for fresh parasite isolates. We explored the possibility of using parasite isolates and reticulocytes, both cryopreserved, to perform invasion and initiate short-term culture. Invasion results obtained with both cryopreserved isolates and reticulocytes were similar to those obtained with fresh samples. This method should be easily replicated in laboratories outside endemic areas and will substantially contribute to the development of a continuous P. vivax culture. In addition, this model could be used for testing vaccine candidates as well as for studying invasion-specific molecular mechanisms. © 2012 Australian Society for Parasitology Inc. |
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Prins Leopold Instituut voor Tropische Geneeskunde |
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Prins Leopold Instituut voor Tropische Geneeskunde Céline Borlon Bruce Russell Kanlaya Sriprawat Rossarin Suwanarusk Annette Erhart Laurent Renia François Nosten Umberto D'Alessandro |
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Article |
author |
Céline Borlon Bruce Russell Kanlaya Sriprawat Rossarin Suwanarusk Annette Erhart Laurent Renia François Nosten Umberto D'Alessandro |
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Céline Borlon |
title |
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
title_short |
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
title_full |
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
title_fullStr |
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
title_full_unstemmed |
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
title_sort |
cryopreserved plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
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2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/14359 |
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1763495317322334208 |