In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.

Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in the patients who have the tumor with soft tissue invasion is rather high. Use of strong chemicals such as phenol and liquid nitrogen to lessen the recurrent rate might not be used in this lesion. The experiment was...

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Main Authors: Rapin Phimolsarnti, Chris Charoenlap, Banjobe Ariyaboonsiri, Adisak Wongkajornsilpa, Saranatra Waikakul
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/14639
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spelling th-mahidol.146392018-06-11T12:04:55Z In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report. Rapin Phimolsarnti Chris Charoenlap Banjobe Ariyaboonsiri Adisak Wongkajornsilpa Saranatra Waikakul Mahidol University Medicine Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in the patients who have the tumor with soft tissue invasion is rather high. Use of strong chemicals such as phenol and liquid nitrogen to lessen the recurrent rate might not be used in this lesion. The experiment was carried out to discover apoptotic effects of thermoablation on giant cell tumor of bone to find out a possibility to use thermoablation in the clinic in the patients with extensive lesion. Tumor cell suspension was prepared from 4 patients who had definite diagnosis of giant cell tumor of bone. The tumor cells were subjected to thermoablation at 45, 47 and 50 degrees C for 10 to 30 minutes before were cultured at 37 degrees C for another 3 days. Osteoblasts and chondrocytes from the last 3 patients were collected,prepared and underwent thermoablation in the same fashion. Apoptosis of tumor cells, chondrocytes and osteoblasts were carried out by the use of flow cytometry. Thermoablation at 47 degrees C for 30 minutes resulted in < 50% chondrocyte and osteoblast apoptosis and 70-90% tumor cells apoptosis of 3/4 patients. Thermoablation at 47 and 50 degrees C for 20 to 30 minutes has more negative effect on giant cell tumor of bone than chondrocytes and osteoblasts. Thermoablation might be a useful tool for local tumor control in the clinic. 2018-06-11T05:04:55Z 2018-06-11T05:04:55Z 2012-09-01 Article Journal of the Medical Association of Thailand = Chotmaihet thangphaet. Vol.95 Suppl 9, (2012) 01252208 2-s2.0-84873548959 https://repository.li.mahidol.ac.th/handle/123456789/14639 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84873548959&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Rapin Phimolsarnti
Chris Charoenlap
Banjobe Ariyaboonsiri
Adisak Wongkajornsilpa
Saranatra Waikakul
In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
description Giant cell tumor of bone is a common benign aggressive bone tumor. Recurrent rate in the patients who have the tumor with soft tissue invasion is rather high. Use of strong chemicals such as phenol and liquid nitrogen to lessen the recurrent rate might not be used in this lesion. The experiment was carried out to discover apoptotic effects of thermoablation on giant cell tumor of bone to find out a possibility to use thermoablation in the clinic in the patients with extensive lesion. Tumor cell suspension was prepared from 4 patients who had definite diagnosis of giant cell tumor of bone. The tumor cells were subjected to thermoablation at 45, 47 and 50 degrees C for 10 to 30 minutes before were cultured at 37 degrees C for another 3 days. Osteoblasts and chondrocytes from the last 3 patients were collected,prepared and underwent thermoablation in the same fashion. Apoptosis of tumor cells, chondrocytes and osteoblasts were carried out by the use of flow cytometry. Thermoablation at 47 degrees C for 30 minutes resulted in < 50% chondrocyte and osteoblast apoptosis and 70-90% tumor cells apoptosis of 3/4 patients. Thermoablation at 47 and 50 degrees C for 20 to 30 minutes has more negative effect on giant cell tumor of bone than chondrocytes and osteoblasts. Thermoablation might be a useful tool for local tumor control in the clinic.
author2 Mahidol University
author_facet Mahidol University
Rapin Phimolsarnti
Chris Charoenlap
Banjobe Ariyaboonsiri
Adisak Wongkajornsilpa
Saranatra Waikakul
format Article
author Rapin Phimolsarnti
Chris Charoenlap
Banjobe Ariyaboonsiri
Adisak Wongkajornsilpa
Saranatra Waikakul
author_sort Rapin Phimolsarnti
title In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
title_short In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
title_full In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
title_fullStr In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
title_full_unstemmed In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
title_sort in vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report.
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/14639
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