Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.

Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.

A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross re...

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Main Authors: V. Boonsaeng, K. Chansiri, P. Vilasineekul, P. Wilairat, S. Panyim
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/15783
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Institution: Mahidol University
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spelling th-mahidol.157832018-06-14T16:14:50Z Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application. V. Boonsaeng K. Chansiri P. Vilasineekul P. Wilairat S. Panyim Mahidol University Medicine A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood. 2018-06-14T09:14:50Z 2018-06-14T09:14:50Z 1989-12-01 Article The Southeast Asian journal of tropical medicine and public health. Vol.20, No.4 (1989), 519-522 01251562 2-s2.0-0024797308 https://repository.li.mahidol.ac.th/handle/123456789/15783 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0024797308&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
V. Boonsaeng
K. Chansiri
P. Vilasineekul
P. Wilairat
S. Panyim
Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
description A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
author2 Mahidol University
author_facet Mahidol University
V. Boonsaeng
K. Chansiri
P. Vilasineekul
P. Wilairat
S. Panyim
format Article
author V. Boonsaeng
K. Chansiri
P. Vilasineekul
P. Wilairat
S. Panyim
author_sort V. Boonsaeng
title Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
title_short Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
title_full Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
title_fullStr Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
title_full_unstemmed Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
title_sort detection of plasmodium falciparum using a cloned dna probe: a simple procedure suitable for field application.
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/15783
_version_ 1763490443243290624

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