Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis

Indirect hemagglutination (IHA) is commonly used for serodiagnosis of melioidosis. However, in endemic areas, high background titers in normal populations and occasional low titers in patients with septicemic melioidosis prompted a search for a more sensitive and more specific method of serodiagnosi...

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Main Authors: M. Kunakorn, P. Boonma, K. Khupulsup, B. Petchclai
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/15957
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spelling th-mahidol.159572018-06-14T16:23:18Z Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis M. Kunakorn P. Boonma K. Khupulsup B. Petchclai Mahidol University Immunology and Microbiology Medicine Indirect hemagglutination (IHA) is commonly used for serodiagnosis of melioidosis. However, in endemic areas, high background titers in normal populations and occasional low titers in patients with septicemic melioidosis prompted a search for a more sensitive and more specific method of serodiagnosis. An indirect fluorescent-antibody test for immunoglobulin M (IgM) specific antibody to Pseudomonas pseudomallei was more sensitive and more specific, but fluorescence microscopes are rarely available in the endemic areas. An enzyme-linked immunosorbent assay (ELISA) for IgM antibody is an attractive alternative. An indirect ELISA for IgM antibody (IgM ELISA) and an IgM antibody capture ELISA for melioidosis were developed. Both tests, together with IHA, were evaluated for 153 serum specimens from blood donors and 16 serum specimens from 16 melioidosis patients. It was found that IHA, the IgM ELISA, and the IgM antibody capture ELISA had sensitivities of 88, 88, and 75%, respectively, with specificities of 97.4, 92.2, and 91.5%, respectively. When IHA was combined with IgM ELISA, a sensitivity of 100% and a specificity of 95.4% were obtained. The IgM ELISA and IHA should be used in combination for serodiagnosis of melioidosis. 2018-06-14T09:21:23Z 2018-06-14T09:21:23Z 1990-06-18 Article Journal of Clinical Microbiology. Vol.28, No.6 (1990), 1249-1253 00951137 2-s2.0-0025287841 https://repository.li.mahidol.ac.th/handle/123456789/15957 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025287841&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
M. Kunakorn
P. Boonma
K. Khupulsup
B. Petchclai
Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
description Indirect hemagglutination (IHA) is commonly used for serodiagnosis of melioidosis. However, in endemic areas, high background titers in normal populations and occasional low titers in patients with septicemic melioidosis prompted a search for a more sensitive and more specific method of serodiagnosis. An indirect fluorescent-antibody test for immunoglobulin M (IgM) specific antibody to Pseudomonas pseudomallei was more sensitive and more specific, but fluorescence microscopes are rarely available in the endemic areas. An enzyme-linked immunosorbent assay (ELISA) for IgM antibody is an attractive alternative. An indirect ELISA for IgM antibody (IgM ELISA) and an IgM antibody capture ELISA for melioidosis were developed. Both tests, together with IHA, were evaluated for 153 serum specimens from blood donors and 16 serum specimens from 16 melioidosis patients. It was found that IHA, the IgM ELISA, and the IgM antibody capture ELISA had sensitivities of 88, 88, and 75%, respectively, with specificities of 97.4, 92.2, and 91.5%, respectively. When IHA was combined with IgM ELISA, a sensitivity of 100% and a specificity of 95.4% were obtained. The IgM ELISA and IHA should be used in combination for serodiagnosis of melioidosis.
author2 Mahidol University
author_facet Mahidol University
M. Kunakorn
P. Boonma
K. Khupulsup
B. Petchclai
format Article
author M. Kunakorn
P. Boonma
K. Khupulsup
B. Petchclai
author_sort M. Kunakorn
title Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
title_short Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
title_full Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
title_fullStr Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
title_full_unstemmed Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
title_sort enzyme-linked immunosorbent assay for immunoglobulin m specific antibody for the diagnosis of melioidosis
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/15957
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