Diagnosis of Plasmodium vivax malaria using a specific deoxyribonucleic acid probe

Diagnosis of Plasmodium vivax malaria using a specific deoxyribonucleic acid probe

A deoxyribonucleic acid (DNA) probe which specifically distinguishes Plasmodium vivax from P. falciparum malaria has been derived from a P. vivax genomic DNA library. This probe, VPL101, consists of 3A 2 kilobase pairs and does not hybridize with up to 6 Ig of human or P. falciparum DNA. VPL101 cont...

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Bibliographic Details
Main Authors: Wendy A. Relf, Robyn E. Boreham, Pramuan Tapchaisri, Srisin Khusmith, Andrew Healey, Peter Upcroft, Savanat Tharavanij, Chev Kidson
Other Authors: Queensland Institute of Medical Research
Format: Article
Published: 2018
Subjects:
Immunology and Microbiology
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/15971
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Institution: Mahidol University
Description
Summary:A deoxyribonucleic acid (DNA) probe which specifically distinguishes Plasmodium vivax from P. falciparum malaria has been derived from a P. vivax genomic DNA library. This probe, VPL101, consists of 3A 2 kilobase pairs and does not hybridize with up to 6 Ig of human or P. falciparum DNA. VPL101 contains at least two copies of a 205 base pair repeat sequence. The subcloned repeat probe, VPL101 5, reacted with 73 of 76 microscopically diagnosed P. vivax samples but not with any of 17 human DNA samples or any of 8 P. falciparum DNA samples from cultured parasites. It was possible to detect P. vivax in mixed infections in which only P. falciparum parasites were identifiable by microscopy. This P. vivax DNA probe provides a useful epidemiological tool for malaria control programmes. © 1990, Royal Society of Tropical Medicine and Hygiene. All Rights Reserved.

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