Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/expl...

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Main Authors: S. Prathanturarug, N. Soonthornchareonnon, W. Chuakul, Y. Phaidee, P. Saralamp
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/16220
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spelling th-mahidol.162202018-06-21T15:06:44Z Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium S. Prathanturarug N. Soonthornchareonnon W. Chuakul Y. Phaidee P. Saralamp Mahidol University Agricultural and Biological Sciences Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production. 2018-06-21T08:06:44Z 2018-06-21T08:06:44Z 2005-03-01 Article Plant Cell, Tissue and Organ Culture. Vol.80, No.3 (2005), 347-351 10.1007/s11240-004-1020-x 01676857 2-s2.0-13244275174 https://repository.li.mahidol.ac.th/handle/123456789/16220 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=13244275174&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
S. Prathanturarug
N. Soonthornchareonnon
W. Chuakul
Y. Phaidee
P. Saralamp
Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
description Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production.
author2 Mahidol University
author_facet Mahidol University
S. Prathanturarug
N. Soonthornchareonnon
W. Chuakul
Y. Phaidee
P. Saralamp
format Article
author S. Prathanturarug
N. Soonthornchareonnon
W. Chuakul
Y. Phaidee
P. Saralamp
author_sort S. Prathanturarug
title Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
title_short Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
title_full Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
title_fullStr Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
title_full_unstemmed Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
title_sort rapid micropropagation of curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/16220
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