L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility
Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive o...
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th-mahidol.16552023-04-12T15:41:17Z L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility Manee-In S Parmornsupornvichit S Kraiprayoon S Tharasanit T Chanapiwat P Kaeoket K Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health Medicine L-carnitine Epididymal Spermatozoa Cryopreservation Cat Open Access article Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examined 2015-02-24T10:50:14Z 2017-04-07T11:55:45Z 2015-02-24T10:50:14Z 2017-04-07T11:55:45Z 2015-02-24 2014-06 Article Asian Australas J Anim Sci. Vol.27, No.6 (Jun 2014), 791-796 10.5713/ajas.2013.13565 1011-2367 https://repository.li.mahidol.ac.th/handle/123456789/1655 eng Mahidol University Asian-Australasian Journal of Animal Sciences application/pdf |
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L-carnitine Epididymal Spermatozoa Cryopreservation Cat Open Access article Manee-In S Parmornsupornvichit S Kraiprayoon S Tharasanit T Chanapiwat P Kaeoket K L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
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Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examined |
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Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health Medicine |
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Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health Medicine Manee-In S Parmornsupornvichit S Kraiprayoon S Tharasanit T Chanapiwat P Kaeoket K |
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Article |
author |
Manee-In S Parmornsupornvichit S Kraiprayoon S Tharasanit T Chanapiwat P Kaeoket K |
author_sort |
Manee-In S |
title |
L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
title_short |
L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
title_full |
L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
title_fullStr |
L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
title_full_unstemmed |
L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
title_sort |
l-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility |
publishDate |
2015 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/1655 |
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1781416825499680768 |