Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay
We developed a novel Plasmodium falciparum genotyping strategy based on the heteroduplex tracking assay (HTA) method commonly used to genotype viruses. Because it can detect both sequence and size polymorphisms, we hypothesized that HTA is more sensitive than current methods. To test this hypothesis...
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th-mahidol.165812018-06-21T15:27:24Z Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay Warunee Ngrenngarmlert Jesse J. Kwiek Deborah D. Kamwendo Kimberly Ritola Ronald Swanstrom Chansuda Wongsrichanalai R. Scott Miller Wanida Ittarat Steven R. Meshnick Mahidol University The University of North Carolina at Chapel Hill University of Michigan School of Public Health Armed Forces Research Institute of Medical Sciences, Thailand Immunology and Microbiology Medicine We developed a novel Plasmodium falciparum genotyping strategy based on the heteroduplex tracking assay (HTA) method commonly used to genotype viruses. Because it can detect both sequence and size polymorphisms, we hypothesized that HTA is more sensitive than current methods. To test this hypothesis, we compared the ability of HTA and a nested polymerase chain reaction (PCR) to detect genetic diversity in 17 Thai samples. The HTA detected more MSP1 sequence variants in eight isolates (47%), less sequence variants in three isolates (18%), and an equal number of sequence variants in six isolates (35%), suggesting that HTA is equal to or more sensitive than the nested PCR. This study is a proof of concept that HTA is a sensitive allelic discrimination method able to determine genetic diversity in P. falciparum and warrants its use in studies of antimalarial drug efficacy. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene. 2018-06-21T08:16:10Z 2018-06-21T08:16:10Z 2005-06-01 Article American Journal of Tropical Medicine and Hygiene. Vol.72, No.6 (2005), 694-701 00029637 2-s2.0-22244475520 https://repository.li.mahidol.ac.th/handle/123456789/16581 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=22244475520&origin=inward |
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Immunology and Microbiology Medicine Warunee Ngrenngarmlert Jesse J. Kwiek Deborah D. Kamwendo Kimberly Ritola Ronald Swanstrom Chansuda Wongsrichanalai R. Scott Miller Wanida Ittarat Steven R. Meshnick Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay |
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We developed a novel Plasmodium falciparum genotyping strategy based on the heteroduplex tracking assay (HTA) method commonly used to genotype viruses. Because it can detect both sequence and size polymorphisms, we hypothesized that HTA is more sensitive than current methods. To test this hypothesis, we compared the ability of HTA and a nested polymerase chain reaction (PCR) to detect genetic diversity in 17 Thai samples. The HTA detected more MSP1 sequence variants in eight isolates (47%), less sequence variants in three isolates (18%), and an equal number of sequence variants in six isolates (35%), suggesting that HTA is equal to or more sensitive than the nested PCR. This study is a proof of concept that HTA is a sensitive allelic discrimination method able to determine genetic diversity in P. falciparum and warrants its use in studies of antimalarial drug efficacy. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene. |
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Mahidol University |
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Mahidol University Warunee Ngrenngarmlert Jesse J. Kwiek Deborah D. Kamwendo Kimberly Ritola Ronald Swanstrom Chansuda Wongsrichanalai R. Scott Miller Wanida Ittarat Steven R. Meshnick |
format |
Article |
author |
Warunee Ngrenngarmlert Jesse J. Kwiek Deborah D. Kamwendo Kimberly Ritola Ronald Swanstrom Chansuda Wongsrichanalai R. Scott Miller Wanida Ittarat Steven R. Meshnick |
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Warunee Ngrenngarmlert |
title |
Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay |
title_short |
Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay |
title_full |
Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay |
title_fullStr |
Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay |
title_full_unstemmed |
Measuring allelic heterogeneity in Plasmodium falciparum by a heteroduplex tracking assay |
title_sort |
measuring allelic heterogeneity in plasmodium falciparum by a heteroduplex tracking assay |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/16581 |
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1763496585262530560 |