Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro
This in vitro study was conducted to assess the blood schizontocidal activity of Desbutyl-benflumetol (DBB), a new benzindene derivative, retinol and a combination of both compounds. The tests were carried out according to the methodology of the WHO standard test Mark II, measuring the drug-dependan...
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th-mahidol.171132018-06-21T15:31:43Z Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro Doris Samal Chaiporn Rojanawatsirivet Gunther Wernsdorfer Herwig Kollaritsch Jeeraphat Sirichaisinthop Walther H. Wernsdorfer Medizinische Universitat Wien Thailand Ministry of Public Health Mahidol University Medicine This in vitro study was conducted to assess the blood schizontocidal activity of Desbutyl-benflumetol (DBB), a new benzindene derivative, retinol and a combination of both compounds. The tests were carried out according to the methodology of the WHO standard test Mark II, measuring the drug-dependant inhibition of schizont maturation, and using 43 fresh isolates of Plasmodium falciparum from northwestern Thailand, an area with established multidrug-resistance. DBB and retinol showed a mean 50% effective concentration (EC-50) of 3.73 nM and 466, 86 nM and 90% effective concentration (EC-90) of 19.83 nM and 5531, 69 nM respectively. The combination of DBB and 3.50 ìM retinol showed strong inhibition of schizont maturation, with an EC-90 for DBB of 0.67 nM. At the therapeutically relevant EC-99, the combination was about 10 times more effective than expected, suggesting that retinol potentiated the effect of DBB. A concentration of 3.50 ìM retinol corresponds to the 95th percentile of the physiological serum levels. It is well known that retinol levels are significantly decreased in acute falciparum malaria. Supplementation with retinol during malaria treatment may improve the therapeutic results of blood schizontocides of the fluorene class. ©2005 Springer-Verlag. 2018-06-21T08:31:43Z 2018-06-21T08:31:43Z 2005-01-01 Article Wiener Klinische Wochenschrift. Vol.117, No.SUPPL. 4 (2005), 39-44 10.1007/s00508-005-0446-4 16137671 00435325 2-s2.0-33644834700 https://repository.li.mahidol.ac.th/handle/123456789/17113 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33644834700&origin=inward |
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Medicine Doris Samal Chaiporn Rojanawatsirivet Gunther Wernsdorfer Herwig Kollaritsch Jeeraphat Sirichaisinthop Walther H. Wernsdorfer Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro |
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This in vitro study was conducted to assess the blood schizontocidal activity of Desbutyl-benflumetol (DBB), a new benzindene derivative, retinol and a combination of both compounds. The tests were carried out according to the methodology of the WHO standard test Mark II, measuring the drug-dependant inhibition of schizont maturation, and using 43 fresh isolates of Plasmodium falciparum from northwestern Thailand, an area with established multidrug-resistance. DBB and retinol showed a mean 50% effective concentration (EC-50) of 3.73 nM and 466, 86 nM and 90% effective concentration (EC-90) of 19.83 nM and 5531, 69 nM respectively. The combination of DBB and 3.50 ìM retinol showed strong inhibition of schizont maturation, with an EC-90 for DBB of 0.67 nM. At the therapeutically relevant EC-99, the combination was about 10 times more effective than expected, suggesting that retinol potentiated the effect of DBB. A concentration of 3.50 ìM retinol corresponds to the 95th percentile of the physiological serum levels. It is well known that retinol levels are significantly decreased in acute falciparum malaria. Supplementation with retinol during malaria treatment may improve the therapeutic results of blood schizontocides of the fluorene class. ©2005 Springer-Verlag. |
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Medizinische Universitat Wien |
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Medizinische Universitat Wien Doris Samal Chaiporn Rojanawatsirivet Gunther Wernsdorfer Herwig Kollaritsch Jeeraphat Sirichaisinthop Walther H. Wernsdorfer |
format |
Article |
author |
Doris Samal Chaiporn Rojanawatsirivet Gunther Wernsdorfer Herwig Kollaritsch Jeeraphat Sirichaisinthop Walther H. Wernsdorfer |
author_sort |
Doris Samal |
title |
Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro |
title_short |
Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro |
title_full |
Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro |
title_fullStr |
Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro |
title_full_unstemmed |
Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro |
title_sort |
synergism of desbutyl-benflumetol and retinol against plasmodium falciparum in vitro |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/17113 |
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1763492984145313792 |