A high performance liquid chromatography system for quantification of hydroxyl radical formation by determination of dihydroxy benzoic acids

• Main Authors: R. W. Owen, T. Wimonwatwatee, B. Spiegelhalder, H. Bartsch
• Other Authors: German Cancer Research Center
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/17535
• Institution: Mahidol University

The hypoxanthine/xanthine oxidase enzyme system is known to produce the superoxide ion and hydrogen peroxide during the hydroxylation of hypoxanthine via xanthine to uric acid. When chelated iron is included in this system, superoxide reduces iron(III) to iron(II) and the iron(II)-chelate further re...