Cloning and nucleotide sequencing of a lipase gene from Bacillus subtilis WRRL-B558
A lipase gene from B. subtilis WRRL-B558 was cloned in Escherichia coli JM109 using pBluescript as a vector plasmid. Two methods were combined to screen for the lipase-producing clone. The first was done by overlaying the screening plates with β-naphthylacetate and Fast Blue BB dye. Positive clones...
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| Main Authors: | , , |
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| Format: | Article |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/17548 |
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| Institution: | Mahidol University |
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