Purification and characterization of lipase from a raw-milk yeast (Trichosporon asteroides)

• Main Authors: Saovanee Dharmsthiti, Palanee Ammaranond
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Engineering; Immunology and Microbiology; Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/17878

A lipase-producing yeast strain was isolated from raw milk. It was identified as Trichosporon asteroides strain LP005. The lipase from this yeast was purified 8-fold to homogeneity for further characterization. The purification process for this lipase included (NH4)2S04 precipitation at 70% saturati...