Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate
Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using...
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| Main Authors: | , |
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| Format: | Article |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/17893 |
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| Institution: | Mahidol University |
| Summary: | Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjunction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for α-anomer of galactoside with an aromatic residue. |
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