Efficient transcription of the larvicidal cry4Ba gene from Bacillus thuringiensis in transgenic chloroplasts of the green algal Chlamydomonas reinhardtii

• Main Authors: Thanate Juntadech, Kittisak Yokthongwattana, Sithichoke Tangphatsornruang, Yun-kiam Yap, Gerd Katzenmeier, Chanan Angsuthanasombat
• Other Authors: Mahidol University. DivisionInstitute of Molecular Biosciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: Chlamydomonas reinhardtii; Chloroplast Transformation; Inverted Repeats; Bt-cry4Ba Transcript; psbA Promoter; Open Access article
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/1804
• Institution: Mahidol University

Unicellular micro-alga Chlamydomonas reinhardtii has been recognized as a promising host for expressing recombinant proteins albeit its limited utility due to low levels of heterologous protein expression. Here, transcription of the 3.4-kb mosquito-larvicidal cry4Ba gene from Bacillus thuringiensis...