Isoleucine at position 150 of Cyt2Aa toxin from Bacillus thuringiensis plays an important role during membrane binding and oligomerization

• Main Authors: Wanwarang Pathaichindachote, Amporn Rungrod, Mongkon Audtho, Sumarin Soonsanga, Chartchai Krittanai, Boonhiang Promdonkoy
• Other Authors: Mahidol University. Institute of Molecular Biosciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: Bacillus thuringiensis; Membrane binding; Mutagenesis; Oligomerization; Open Access article
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/1821
• Institution: Mahidol University

Cyt2Aa2 is a mosquito larvicidal and cytolytic toxin produced by Bacillus thuringiensis subsp. darmstadiensis. The toxin becomes inactive when isoleucine at position 150 was replaced by alanine. To investigate the functional role of this position, Ile150 was substituted with Leu, Phe, Glu and Lys. A...