Hydrogen peroxide toxicity induces ras signaling in human neuroblastoma SH-SY5Y cultured cells

• Main Authors: Jirapa Chetsawang, Piyarat Govitrapong, Banthit Chetsawang
• Other Authors: Mahidol University. Institute of Molecular Biosciences. Research Center for Neuroscience
• Format: Article
• Language: English
• Published: 2015
• Subjects: Hydrogen Peroxide; Toxicity Induces; Ras Signaling; Human Neuroblastoma; SH-SY5Y; Cultured Cells; Open Access article
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/1830
• Institution: Mahidol University

It has been reported that overproduction of reactive oxygen species occurs after brain injury and mediates neuronal cells degeneration. In the present study, we examined the role of Ras signaling on hydrogen peroxide-induced neuronal cells degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y cultured cells. An inhibitor of the enzyme that catalyzes the farnesylation of Ras proteins, FTI-277, and a competitive inhibitor of GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y cultured cells. The results of this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen peroxideinduced toxicity in neuronal cells.