Validation of a reference gene for transcript analysis in cassava (Manihot esculenta Crantz) and its application in analysis of linamarase and α-hydroxynitrile lyase expression at different growth stages

• Main Authors: Sukhuman Whankaew, Supajit Sraphet, Ratchadaporn Thaikert, Opas Boonseng, Duncan R. Smith, Kanokporn Triwitayakorn
• Other Authors: Mahidol University. Institute of Molecular Biosciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: Cassava; housekeeping gene; hydroxynitrile lyase; linamarase; real-time polymerase chain reaction (PCR); Open Access article
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/1838
• Institution: Mahidol University

Relative real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a well-established method for the precise quantification of gene expression. For accurate relative real-time RT-qPCR analysis, validation of the expression of an appropriate reference gene is required. In th...