Qualitative detection of avian influenza A (H5N1) viruses: A comparative evaluation of four real-time nucleic acid amplification methods

• Main Authors: Wasun Chantratita, Chonlaphat Sukasem, Supaporn Kaewpongsri, Chutatip Srichunrusami, Wantanit Pairoj, Arunee Thitithanyanont, Kridsada Chaichoune, Parntep Ratanakron, Thaweesak Songserm, Sudarat Damrongwatanapokin, Olfert Landt
• Other Authors: Faculty of Medicine, Thammasat University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/18847
• Institution: Mahidol University

The aim of this study was to determine the performance of real-time amplification based methods - NASBA, TaqMan, RT-FRET, and RT-PCR LUX™ formats - for the detection of influenza A (H5N1) virus RNA. In an analysis of 54 samples obtained from a range of animal species in Thailand during the period 20...