NADPH-cytochrome P450 oxidoreductase from the mosquito Anopheles minimus: Kinetic studies and the influence of Leu86 and Leu219 on cofactor binding and protein stability
NADPH-cytochrome c oxidoreductase from the mosquito Anopheles minimus lacking the first 55 amino acid residues was expressed in Escherichia coli. The purified enzyme loses FMN, leading to an unstable protein and subsequent aggregation. To understand the basis for the instability, we constructed sing...
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| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/18864 |
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| Institution: | Mahidol University |