New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients
Background: Absolute CD4+ T-lymphocyte counts are used in the initiation and monitoring of antiretroviral therapy in HIV-infected patients. Becton Dickinson's (BD) FACSCount™ system was introduced 12 years ago as a dedicated instrument for enumeration of absolute CD4+ T-lymphocytes. However, th...
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th-mahidol.189362018-07-12T09:43:59Z New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients Kovit Pattanapanyasat Kasama Sukapirom Laddawan Kowawisatsut Charin Thepthai Mahidol University Biochemistry, Genetics and Molecular Biology Medicine Background: Absolute CD4+ T-lymphocyte counts are used in the initiation and monitoring of antiretroviral therapy in HIV-infected patients. Becton Dickinson's (BD) FACSCount™ system was introduced 12 years ago as a dedicated instrument for enumeration of absolute CD4+ T-lymphocytes. However, this system does not provide percent CD4+ T-lymphocyte that is the required monitoring parameter in pediatric patients. We evaluated a new BD FACSCount CD4 software and reagents for simultaneous percent and absolute CD4+ T-lymphocytes in HIV-infected blood. Methods: Percent and absolute CD4+ T-lymphocytes in 149 HIV-infected blood samples were determined using a new FACSCount system. Results of percent and absolute CD4+ T-lymphocytes were compared between the dual-platform (DP) method, using BD FACScan™ flow cytometer plus hematology analyzer and the standard FACSCount system. Correlation and agreement were analyzed using linear regression and Bland-Altman analysis. Results: Percent CD4+ T-lymphocyte values obtained from the new FACSCount system correlated well with DP FACScan method (r2= 0.977, P < 0.0001). Mean bias was only -0.36% [limit of agreement (LOA): -2.52% to +1.80%] and percent similarity was 101.36%. For absolute CD4+ T-lymphocyte, the new system correlated highly with standard FACSCount system (r2= 0.986, P < 0.0001), with a percent similarity of 98.2. Mean bias was +3.39 cells/μl with LOA of -52.53 cells/μ to +59.31 cells/μl. Conclusion: This new FACSCount system is a simple and reliable system for enumeration of absolute and percent CD4+ T-lymphocytes. Having one system giving both results should reduce the cost and thus increase access to CD4 testing for pediatric and adult patients. © 2008 Clinical Cytometry Society. 2018-07-12T02:18:58Z 2018-07-12T02:18:58Z 2008-04-28 Article Cytometry Part B - Clinical Cytometry. Vol.74, No.SUPPL. 1 (2008) 10.1002/cyto.b.20415 15524957 15524949 2-s2.0-42449129627 https://repository.li.mahidol.ac.th/handle/123456789/18936 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42449129627&origin=inward |
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Biochemistry, Genetics and Molecular Biology Medicine Kovit Pattanapanyasat Kasama Sukapirom Laddawan Kowawisatsut Charin Thepthai New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients |
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Background: Absolute CD4+ T-lymphocyte counts are used in the initiation and monitoring of antiretroviral therapy in HIV-infected patients. Becton Dickinson's (BD) FACSCount™ system was introduced 12 years ago as a dedicated instrument for enumeration of absolute CD4+ T-lymphocytes. However, this system does not provide percent CD4+ T-lymphocyte that is the required monitoring parameter in pediatric patients. We evaluated a new BD FACSCount CD4 software and reagents for simultaneous percent and absolute CD4+ T-lymphocytes in HIV-infected blood. Methods: Percent and absolute CD4+ T-lymphocytes in 149 HIV-infected blood samples were determined using a new FACSCount system. Results of percent and absolute CD4+ T-lymphocytes were compared between the dual-platform (DP) method, using BD FACScan™ flow cytometer plus hematology analyzer and the standard FACSCount system. Correlation and agreement were analyzed using linear regression and Bland-Altman analysis. Results: Percent CD4+ T-lymphocyte values obtained from the new FACSCount system correlated well with DP FACScan method (r2= 0.977, P < 0.0001). Mean bias was only -0.36% [limit of agreement (LOA): -2.52% to +1.80%] and percent similarity was 101.36%. For absolute CD4+ T-lymphocyte, the new system correlated highly with standard FACSCount system (r2= 0.986, P < 0.0001), with a percent similarity of 98.2. Mean bias was +3.39 cells/μl with LOA of -52.53 cells/μ to +59.31 cells/μl. Conclusion: This new FACSCount system is a simple and reliable system for enumeration of absolute and percent CD4+ T-lymphocytes. Having one system giving both results should reduce the cost and thus increase access to CD4 testing for pediatric and adult patients. © 2008 Clinical Cytometry Society. |
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Mahidol University |
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Mahidol University Kovit Pattanapanyasat Kasama Sukapirom Laddawan Kowawisatsut Charin Thepthai |
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Kovit Pattanapanyasat Kasama Sukapirom Laddawan Kowawisatsut Charin Thepthai |
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Kovit Pattanapanyasat |
title |
New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients |
title_short |
New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients |
title_full |
New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients |
title_fullStr |
New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients |
title_full_unstemmed |
New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients |
title_sort |
new bd facscount™ cd4 reagent system for simultaneous enumeration of percent and absolute cd4 t-lymphocytes in hiv-1-infected pediatric patients |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/18936 |
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1763490824951169024 |