Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans

Burkholderia pseudomallei is the causative agent of melioidosis, a bacterial infection endemic in tropical regions including southeast Asia and northern Australia. B. pseudomallei contains structurally unique polysaccharides (capsular polysaccharide and O-antigen saccharides of lipopolysaccharide)....

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Main Authors: Narayanan Parthasarathy, David DeShazer, Sharon J. Peacock, Vanaporn Wuthiekanun, Marilyn J. England, Sarah L. Norris, David M. Waag
Other Authors: U.S. Army Medical Research Institute of Infectious Diseases
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/18998
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spelling th-mahidol.189982018-07-12T09:23:42Z Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans Narayanan Parthasarathy David DeShazer Sharon J. Peacock Vanaporn Wuthiekanun Marilyn J. England Sarah L. Norris David M. Waag U.S. Army Medical Research Institute of Infectious Diseases Mahidol University Biochemistry, Genetics and Molecular Biology Chemistry Burkholderia pseudomallei is the causative agent of melioidosis, a bacterial infection endemic in tropical regions including southeast Asia and northern Australia. B. pseudomallei contains structurally unique polysaccharides (capsular polysaccharide and O-antigen saccharides of lipopolysaccharide). A polysaccharide microarray platform was developed by immobilizing these polysaccharides onto glass slides. Employing this microarray, we were able to demonstrate the presence of antibodies to these polysaccharide antigens in the sera of melioidosis patients, but not in serum from nonmelioidosis human subjects. The advantages of this polysaccharide microarray technology over the conventional tests for the serodiagnosis of melioidosis are discussed. Copyright © Taylor & Francis Group, LLC. 2018-07-12T02:20:24Z 2018-07-12T02:20:24Z 2008-01-01 Article Journal of Carbohydrate Chemistry. Vol.27, No.1 (2008), 32-40 10.1080/07328300802030761 15322327 07328303 2-s2.0-45949089731 https://repository.li.mahidol.ac.th/handle/123456789/18998 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=45949089731&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Narayanan Parthasarathy
David DeShazer
Sharon J. Peacock
Vanaporn Wuthiekanun
Marilyn J. England
Sarah L. Norris
David M. Waag
Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans
description Burkholderia pseudomallei is the causative agent of melioidosis, a bacterial infection endemic in tropical regions including southeast Asia and northern Australia. B. pseudomallei contains structurally unique polysaccharides (capsular polysaccharide and O-antigen saccharides of lipopolysaccharide). A polysaccharide microarray platform was developed by immobilizing these polysaccharides onto glass slides. Employing this microarray, we were able to demonstrate the presence of antibodies to these polysaccharide antigens in the sera of melioidosis patients, but not in serum from nonmelioidosis human subjects. The advantages of this polysaccharide microarray technology over the conventional tests for the serodiagnosis of melioidosis are discussed. Copyright © Taylor & Francis Group, LLC.
author2 U.S. Army Medical Research Institute of Infectious Diseases
author_facet U.S. Army Medical Research Institute of Infectious Diseases
Narayanan Parthasarathy
David DeShazer
Sharon J. Peacock
Vanaporn Wuthiekanun
Marilyn J. England
Sarah L. Norris
David M. Waag
format Article
author Narayanan Parthasarathy
David DeShazer
Sharon J. Peacock
Vanaporn Wuthiekanun
Marilyn J. England
Sarah L. Norris
David M. Waag
author_sort Narayanan Parthasarathy
title Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans
title_short Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans
title_full Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans
title_fullStr Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans
title_full_unstemmed Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans
title_sort application of polysaccharide microarray technology for the serodiagnosis of burkholderia pseudomallei infection (melioidosis) in humans
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/18998
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