Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates

• Main Authors: Takafumi Tsuboi, Satoru Takeo, Hideyuki Iriko, Ling Jin, Masateru Tsuchimochi, Shusaku Matsuda, Eun Taek Han, Hitoshi Otsuki, Osamu Kaneko, Jetsumon Sattabongkot, Rachanee Udomsangpetch, Tatsuya Sawasaki, Motomi Torii, Yaeta Endo
• Other Authors: Ehime University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/19348
• Institution: Mahidol University

One of the major bottlenecks in malaria research has been the difficulty in recombinant protein expression. Here, we report the application of the wheat germ cell-free system for the successful production of malaria proteins. For proof of principle, the Pfs25, PfCSP, and PfAMA1 proteins were chosen....