Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings

Vitamin A deficiency (VAD) is a significant public health problem in many countries. While cost-effective interventions are available to control VAD, reliable information is needed to the track progress of control programmes. However, assessment of VAD is uncommon because current approaches are expe...

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Main Authors: Jonathan L. Gorstein, Omar Dary, Pongtorn, Bettina Shell-Duncan, Tim Quick, Emorn Wasanwisut
Other Authors: University of Washington School of Public Health and Community Medicine
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/19695
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spelling th-mahidol.196952018-07-12T09:49:53Z Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings Jonathan L. Gorstein Omar Dary Pongtorn Bettina Shell-Duncan Tim Quick Emorn Wasanwisut University of Washington School of Public Health and Community Medicine A2Z Project Mahidol University United States Agency for International Development Medicine Nursing Vitamin A deficiency (VAD) is a significant public health problem in many countries. While cost-effective interventions are available to control VAD, reliable information is needed to the track progress of control programmes. However, assessment of VAD is uncommon because current approaches are expensive and not feasible in low-resource settings. The present study explores the utility of retinol-binding protein (RBP), analysed by enzyme-linked immunosorbent assay from capillary blood, as an alternative measure of serum retinol concentrations in populations. The study collected matched panels of venous and capillary blood from pre-school children in Chiang Mai, Thailand. Of a total sample of 195 children, there were no differences between RBP from venous blood, RBP from capillary blood or retinol from capillary blood relative to retinol from venous blood. Receiver-operating characteristic curve analysis suggested a cut-off of RBP < 0.825 μmol l-1had optimal screening proficiency relative to retinol <0.70 μmol l-1. For the purpose of population assessment, all three parameters performed well in screening for VAD relative to retinol from venous blood. There were no differences in the estimates of VAD between children stratified by inflammation status. Lower RBP concentrations were found in children in the early convalescent stage of infection than in children with no infection or in the late convalescent stage. This study provided evidence of the biological comparability between retinol and RBP estimated from venous blood and capillary blood. This is a critical observation as it provides empirical evidence that RBP from capillary blood is a surrogate measure of serum retinol concentrations. © 2007 The Authors. 2018-07-12T02:43:53Z 2018-07-12T02:43:53Z 2008-05-01 Article Public Health Nutrition. Vol.11, No.5 (2008), 513-520 10.1017/S1368980007000821 14752727 13689800 2-s2.0-42149121968 https://repository.li.mahidol.ac.th/handle/123456789/19695 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42149121968&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
Nursing
spellingShingle Medicine
Nursing
Jonathan L. Gorstein
Omar Dary
Pongtorn
Bettina Shell-Duncan
Tim Quick
Emorn Wasanwisut
Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
description Vitamin A deficiency (VAD) is a significant public health problem in many countries. While cost-effective interventions are available to control VAD, reliable information is needed to the track progress of control programmes. However, assessment of VAD is uncommon because current approaches are expensive and not feasible in low-resource settings. The present study explores the utility of retinol-binding protein (RBP), analysed by enzyme-linked immunosorbent assay from capillary blood, as an alternative measure of serum retinol concentrations in populations. The study collected matched panels of venous and capillary blood from pre-school children in Chiang Mai, Thailand. Of a total sample of 195 children, there were no differences between RBP from venous blood, RBP from capillary blood or retinol from capillary blood relative to retinol from venous blood. Receiver-operating characteristic curve analysis suggested a cut-off of RBP < 0.825 μmol l-1had optimal screening proficiency relative to retinol <0.70 μmol l-1. For the purpose of population assessment, all three parameters performed well in screening for VAD relative to retinol from venous blood. There were no differences in the estimates of VAD between children stratified by inflammation status. Lower RBP concentrations were found in children in the early convalescent stage of infection than in children with no infection or in the late convalescent stage. This study provided evidence of the biological comparability between retinol and RBP estimated from venous blood and capillary blood. This is a critical observation as it provides empirical evidence that RBP from capillary blood is a surrogate measure of serum retinol concentrations. © 2007 The Authors.
author2 University of Washington School of Public Health and Community Medicine
author_facet University of Washington School of Public Health and Community Medicine
Jonathan L. Gorstein
Omar Dary
Pongtorn
Bettina Shell-Duncan
Tim Quick
Emorn Wasanwisut
format Article
author Jonathan L. Gorstein
Omar Dary
Pongtorn
Bettina Shell-Duncan
Tim Quick
Emorn Wasanwisut
author_sort Jonathan L. Gorstein
title Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
title_short Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
title_full Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
title_fullStr Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
title_full_unstemmed Feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
title_sort feasibility of using retinol-binding protein from capillary blood specimens to estimate serum retinol concentrations and the prevalence of vitamin a deficiency in low-resource settings
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/19695
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