SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia

The Anopheles dirus Peyton & Harrison complex of mosquitoes (Diptera: Culicidae) comprises seven known species, including important malaria vectors in Southeast Asia. Specific identification of each species of the complex, which cannot be distinguished using morphological characters, is crucial...

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Main Authors:	Sylvie Manguin, P. Kengne, L. Sonnier, R. E. Harbach, V. Baimai, H. D. Trung, M. Coosemans
Other Authors:	Centre de Biologie pour la Gestion des Populations
Format:	Article
Published:	2018
Subjects:	Agricultural and Biological Sciences Immunology and Microbiology Veterinary
Online Access:	https://repository.li.mahidol.ac.th/handle/123456789/20013
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Institution:	Mahidol University

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spelling	th-mahidol.200132018-07-24T10:13:18Z SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia Sylvie Manguin P. Kengne L. Sonnier R. E. Harbach V. Baimai H. D. Trung M. Coosemans Centre de Biologie pour la Gestion des Populations IRD Institut de Recherche pour le Developpement CNRS Centre National de la Recherche Scientifique The Natural History Museum, London Mahidol University National Institute of Malariology, Parasitology and Entomology Hanoi Prins Leopold Instituut voor Tropische Geneeskunde Agricultural and Biological Sciences Immunology and Microbiology Veterinary The Anopheles dirus Peyton & Harrison complex of mosquitoes (Diptera: Culicidae) comprises seven known species, including important malaria vectors in Southeast Asia. Specific identification of each species of the complex, which cannot be distinguished using morphological characters, is crucial for understanding vector ecology and implementing effective control measures. Derived from individual random amplified polymorphic DNA (RAPD) markers, sequence characterized amplified regions (SCAR) were analysed for the design of specific paired-primers. Combination of six SCAR primers resulted in the development of a simple, robust, single multiplex PCR able to identify three important malaria vectors among the four most common species (A, B, C, D) of the complex: species A from several Southeast Asian countries, species B from Perlis, Malaysia, and species C and D from Thailand. 2018-07-24T02:55:47Z 2018-07-24T02:55:47Z 2002-01-01 Article Medical and Veterinary Entomology. Vol.16, No.1 (2002), 46-54 10.1046/j.0269-283x.2002.00344.x 0269283X 2-s2.0-0036010084 https://repository.li.mahidol.ac.th/handle/123456789/20013 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0036010084&origin=inward
institution	Mahidol University
building	Mahidol University Library
continent	Asia
country	Thailand Thailand
content_provider	Mahidol University Library
collection	Mahidol University Institutional Repository
topic	Agricultural and Biological Sciences Immunology and Microbiology Veterinary
spellingShingle	Agricultural and Biological Sciences Immunology and Microbiology Veterinary Sylvie Manguin P. Kengne L. Sonnier R. E. Harbach V. Baimai H. D. Trung M. Coosemans SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
description	The Anopheles dirus Peyton & Harrison complex of mosquitoes (Diptera: Culicidae) comprises seven known species, including important malaria vectors in Southeast Asia. Specific identification of each species of the complex, which cannot be distinguished using morphological characters, is crucial for understanding vector ecology and implementing effective control measures. Derived from individual random amplified polymorphic DNA (RAPD) markers, sequence characterized amplified regions (SCAR) were analysed for the design of specific paired-primers. Combination of six SCAR primers resulted in the development of a simple, robust, single multiplex PCR able to identify three important malaria vectors among the four most common species (A, B, C, D) of the complex: species A from several Southeast Asian countries, species B from Perlis, Malaysia, and species C and D from Thailand.
author2	Centre de Biologie pour la Gestion des Populations
author_facet	Centre de Biologie pour la Gestion des Populations Sylvie Manguin P. Kengne L. Sonnier R. E. Harbach V. Baimai H. D. Trung M. Coosemans
format	Article
author	Sylvie Manguin P. Kengne L. Sonnier R. E. Harbach V. Baimai H. D. Trung M. Coosemans
author_sort	Sylvie Manguin
title	SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
title_short	SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
title_full	SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
title_fullStr	SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
title_full_unstemmed	SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia
title_sort	scar markers and multiplex pcr-based identification of isomorphic species in the anopheles dirus complex in southeast asia
publishDate	2018
url	https://repository.li.mahidol.ac.th/handle/123456789/20013
_version_	1763497807062237184

SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia

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