Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients

Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients

Blood samples were collected from 100 pediatric AIDS patients for the detection of CMV in pp65-bearing leukocytes (PBLs) by immunoperoxidase staining (IP) and PCR. IgM antibody assay was performed to determine the correlation of antigen and antibody. IP and PCR can be used as methods for the early d...

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Main Authors: Surang Tantivanich, Wandee Sawatmongkonkun, Kruavan Balachandra, Tawee Chotpitayasunondh, Pornsawan Amarapal
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/20291
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spelling th-mahidol.202912018-07-24T10:03:21Z Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients Surang Tantivanich Wandee Sawatmongkonkun Kruavan Balachandra Tawee Chotpitayasunondh Pornsawan Amarapal Mahidol University Thailand Ministry of Public Health Queen Sirikit National Institute of Child Health Medicine Blood samples were collected from 100 pediatric AIDS patients for the detection of CMV in pp65-bearing leukocytes (PBLs) by immunoperoxidase staining (IP) and PCR. IgM antibody assay was performed to determine the correlation of antigen and antibody. IP and PCR can be used as methods for the early detection of CMV (prior to the presence of IgM antibody). The sensitivity and specificity of IP were 73% and 97% respectively. IP is superior to PCR in several ways: it is very easy to perform, less time consuming, less expensive, and does not require expensive instruments. 2018-07-24T03:03:21Z 2018-07-24T03:03:21Z 2002-12-01 Article Southeast Asian Journal of Tropical Medicine and Public Health. Vol.33, No.2 (2002), 306-311 01251562 2-s2.0-0036595974 https://repository.li.mahidol.ac.th/handle/123456789/20291 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0036595974&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Surang Tantivanich
Wandee Sawatmongkonkun
Kruavan Balachandra
Tawee Chotpitayasunondh
Pornsawan Amarapal
Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients
description Blood samples were collected from 100 pediatric AIDS patients for the detection of CMV in pp65-bearing leukocytes (PBLs) by immunoperoxidase staining (IP) and PCR. IgM antibody assay was performed to determine the correlation of antigen and antibody. IP and PCR can be used as methods for the early detection of CMV (prior to the presence of IgM antibody). The sensitivity and specificity of IP were 73% and 97% respectively. IP is superior to PCR in several ways: it is very easy to perform, less time consuming, less expensive, and does not require expensive instruments.
author2 Mahidol University
author_facet Mahidol University
Surang Tantivanich
Wandee Sawatmongkonkun
Kruavan Balachandra
Tawee Chotpitayasunondh
Pornsawan Amarapal
format Article
author Surang Tantivanich
Wandee Sawatmongkonkun
Kruavan Balachandra
Tawee Chotpitayasunondh
Pornsawan Amarapal
author_sort Surang Tantivanich
title Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients
title_short Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients
title_full Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients
title_fullStr Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients
title_full_unstemmed Rapid diagnosis of cytomegalovirus in Thai pediatric AIDS patients
title_sort rapid diagnosis of cytomegalovirus in thai pediatric aids patients
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/20291
_version_ 1763490311585136640

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