Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis
The MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis was developed by using bacterial suspension prepared from colonies on solid media. The MTT tube assay in 1 ml Middlebrook 7H9 broth was completed within 4 days for rifampicin (RMP) and within 7 days for...
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th-mahidol.202972018-07-24T10:03:40Z Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis S. Foongladda D. Roengsanthia W. Arjrattanakool C. Chuchottaworn A. Chaiprasert S. G. Franzblau Mahidol University Central Chest Hospital Thailand University of Illinois at Chicago Medicine The MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis was developed by using bacterial suspension prepared from colonies on solid media. The MTT tube assay in 1 ml Middlebrook 7H9 broth was completed within 4 days for rifampicin (RMP) and within 7 days for isoniazid (INH). When MTT assay results with 279 M. tuberculosis clinical isolates were compared with those of the conventional proportion method on Löwenstein-Jensen medium, high specificity and sensitivity values of 100% and 94.1%, respectively, for RMP susceptibility testing, and 99.5% and 89.2%, respectively, for INH susceptibility testing were obtained. The accuracy of the MTT method for RMP and INH was >0.97 concordance with the proportion method. The MTT method is simple, inexpensive and rapid. The high level of agreement with the conventional proportion method suggests a potential to rapidly detect drug-resistant M. tuberculosis in developing countries, as only basic microbiological equipment is need. 2018-07-24T03:03:40Z 2018-07-24T03:03:40Z 2002-12-01 Article International Journal of Tuberculosis and Lung Disease. Vol.6, No.12 (2002), 1118-1122 10273719 2-s2.0-0036924086 https://repository.li.mahidol.ac.th/handle/123456789/20297 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0036924086&origin=inward |
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Medicine S. Foongladda D. Roengsanthia W. Arjrattanakool C. Chuchottaworn A. Chaiprasert S. G. Franzblau Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis |
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The MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis was developed by using bacterial suspension prepared from colonies on solid media. The MTT tube assay in 1 ml Middlebrook 7H9 broth was completed within 4 days for rifampicin (RMP) and within 7 days for isoniazid (INH). When MTT assay results with 279 M. tuberculosis clinical isolates were compared with those of the conventional proportion method on Löwenstein-Jensen medium, high specificity and sensitivity values of 100% and 94.1%, respectively, for RMP susceptibility testing, and 99.5% and 89.2%, respectively, for INH susceptibility testing were obtained. The accuracy of the MTT method for RMP and INH was >0.97 concordance with the proportion method. The MTT method is simple, inexpensive and rapid. The high level of agreement with the conventional proportion method suggests a potential to rapidly detect drug-resistant M. tuberculosis in developing countries, as only basic microbiological equipment is need. |
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Mahidol University |
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Mahidol University S. Foongladda D. Roengsanthia W. Arjrattanakool C. Chuchottaworn A. Chaiprasert S. G. Franzblau |
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S. Foongladda D. Roengsanthia W. Arjrattanakool C. Chuchottaworn A. Chaiprasert S. G. Franzblau |
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S. Foongladda |
title |
Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis |
title_short |
Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis |
title_full |
Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis |
title_fullStr |
Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis |
title_full_unstemmed |
Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis |
title_sort |
rapid and simple mtt method for rifampicin and isoniazid susceptibility testing of mycobacterium tuberculosis |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/20297 |
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1763492290121170944 |