Targeted modification of a human β-globin locus BAC clone using GET Recombination and an I-SceI counterselection cassette

• Main Authors: Duangporn Jamsai, Michael Orford, Mikhail Nefedov, Suthat Fucharoen, Robert Williamson, Panayiotis A. Ioannou
• Other Authors: University of Melbourne
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/20714
• Institution: Mahidol University

There is a need for better approaches to allow precise engineering of large genomic BAC DNA fragments, to facilitate the use of intact genomic loci for therapeutic and biotechnology applications. We report an efficient method to insert any modification in any genomic locus, using a human β-globin lo...