Detection of non-amplified mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors
Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/2072 |
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| Institution: | Mahidol University |
| Language: | English |
| Summary: | Piezoelectric DNA-based biosensor technology was developed as a new method
for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified
oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled
monolayer method. The advantage of this study is that a non-amplified genomic
bacterial DNA target was used. Instead, the genomic DNA was digested by restriction
enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor
was evaluated through an examination of 200 samples. No cross hybridization were
observed against M. avium complex and other microorganisms. This target DNA
preparation, without PCR amplification, will reduce time, costs, and the tedious step
of amplification. |
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