Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen dete...
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th-mahidol.20742023-04-12T15:32:25Z Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection Raweewan Thiramanas Kulachart Jangpatarapongsa Udom Asawapirom Pramuan Tangboriboonrat Duangporn Polpanich Center for Innovation Development and Technology Transfer bovine serum-albumin plasmodium-vivax colloidal stability cross-reactivity proteins antibody immobilization agglutination adsorption falciparum Open Access article Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody (Ab) specific to Plasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasma were investigated. All Ab–latex conjugates provided the high sensitivity for the detection of P. falciparum malaria plasma. The highest specificity to P. falciparum was obtained from using Ab–NPs conjugated via the SA–biotin interaction. 2014-01-14T04:16:47Z 2017-06-20T16:08:13Z 2014-01-14T04:16:47Z 2017-06-20T16:08:13Z 2012-11 Article Microbial biotechnology. Vol.6, No.4 (2013), 406-413 https://repository.li.mahidol.ac.th/handle/123456789/2074 eng Mahidol University Microbial Biotechnology application/pdf |
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bovine serum-albumin plasmodium-vivax colloidal stability cross-reactivity proteins antibody immobilization agglutination adsorption falciparum Open Access article |
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bovine serum-albumin plasmodium-vivax colloidal stability cross-reactivity proteins antibody immobilization agglutination adsorption falciparum Open Access article Raweewan Thiramanas Kulachart Jangpatarapongsa Udom Asawapirom Pramuan Tangboriboonrat Duangporn Polpanich Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection |
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Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody (Ab) specific to Plasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasma were investigated. All Ab–latex conjugates provided the high sensitivity for the detection of P. falciparum malaria plasma. The highest specificity to P. falciparum was obtained from using Ab–NPs conjugated via the SA–biotin interaction. |
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Center for Innovation Development and Technology Transfer |
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Center for Innovation Development and Technology Transfer Raweewan Thiramanas Kulachart Jangpatarapongsa Udom Asawapirom Pramuan Tangboriboonrat Duangporn Polpanich |
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Article |
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Raweewan Thiramanas Kulachart Jangpatarapongsa Udom Asawapirom Pramuan Tangboriboonrat Duangporn Polpanich |
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Raweewan Thiramanas |
title |
Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection |
title_short |
Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection |
title_full |
Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection |
title_fullStr |
Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection |
title_full_unstemmed |
Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection |
title_sort |
sensitivity and specificity of ps/aa-modified nanoparticles used in malaria detection |
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2014 |
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https://repository.li.mahidol.ac.th/handle/123456789/2074 |
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1781415990859399168 |