Cloning of a chitinase gene into Bacillus thuringiensis subsp. aizawai for enhanced insecticidal activity

• Main Authors: Tantimavanich S, Pantuwatana S, Bhumiratana A, Panbangred W
• Other Authors: Faculty of Science. Department of Biotechnology
• Format: Article
• Language: English
• Published: 2015
• Subjects: bacillus licheniformis; bacillus thuringiensis; b.t.a.-toxicity; chitinase; delta-endotoxin; spodoptera exigua; Open Access article
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/2078
• Institution: Mahidol University

Chitinase from a high producing strain (TP-1) of Bacillus licheniformis was used with B. thuringiensis subsp. aizawai (B.t.a.) in a combined larvicidal assay against the pest, Spodoptera exigua. With 10 mU of this chitinase, the LD(50) of B.t.a. was reduced by 7.6, 13.8 and 15 times on days 3, 5 and...