Preparation of spent brewer's yeast β-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon

• Main Authors: M. Suphantharika, P. Khunrae, P. Thanardkit, C. Verduyn
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Chemical Engineering; Energy; Environmental Science
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/20796

Spent brewer's yeast was autolysed and the insoluble yeast cell wall fraction obtained was used as a raw material for the preparation of brewer's yeast β-glucan (BYG). A simple alkaline extraction was applied and optimized. The BYG with significantly (P≤0.05) high carbohydrate and glucan contents of 92% and 51% (w/w), respectively and with a low protein content of 1.6% (w/w) was obtained by a single alkaline extraction using 5 volumes of 1.0 N NaOH at 90 °C for 1 h. In vitro, this glucan significantly (P≤0.05) enhanced phenoloxidase (PO) activity of black tiger shrimp hemolymph as compared to controls without added glucan. Also in vivo, an oral administration of 0.2% (w/w) in diets for 3 days significantly (P≤0.05) increased the PO-activity of the shrimp. The potential immunostimulating properties of a commercial and other yeast-derived products were also evaluated for comparative purposes. © 2002 Elsevier Science Ltd. All rights reserved.