Immobilization of glucose oxidase onto Langmuir-Blodgett films of poly-3-hexylthiophene

Immobilization of glucose oxidase onto Langmuir-Blodgett films of poly-3-hexylthiophene

Monolayers of poly(3-hexyl thiophene) (P3HT) were obtained by dispensing a solution of P3HT with stearic acid (SA) in chloroform at air-water interface using Joyce-Loebl LB trough. These films were transferred onto indium-tin-oxide coated glass plate by vertical dipping method. Enzyme glucose oxidas...

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Bibliographic Details
Main Authors: Rahul Singhal, Asha Chaubey, Toemsak Srikhirin, Sukanya Aphiwantrakul, Shyam S. Pandey, Bansi D. Malhotra
Other Authors: National Physical Laboratory India
Format: Article
Published: 2018
Subjects:
Materials Science
Physics and Astronomy
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/20955
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Institution: Mahidol University
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Summary:Monolayers of poly(3-hexyl thiophene) (P3HT) were obtained by dispensing a solution of P3HT with stearic acid (SA) in chloroform at air-water interface using Joyce-Loebl LB trough. These films were transferred onto indium-tin-oxide coated glass plate by vertical dipping method. Enzyme glucose oxidase (GOX) was immobilized on the films using LB technique. These films were characterized by X-ray diffraction and atomic force microscopic techniques. Photometric response of these P3HT/SA/GOX films was obtained as a function of glucose concentration. These P3HT/SA/GOX LB films show a linearity from 100 to 500 mg/dl of glucose concentration. © 2002 Elsevier Science B.V. All rights reserved.

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