In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand
The acquisition of resistance to chloroquine by Plasmodium vivax in parts of the Australasian and Malaysian epidemiological zones and hitherto unconfirmed reports of such resistance in neighbouring zones show the need for monitoring the drug response of P. vivax. In this study, a recently developed...
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th-mahidol.210022018-07-24T10:28:22Z In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand Daniela Lux Somsak Prajakwong Herwig Kollaritsch Gunther Wernsdorfer Walther H. Wernsdorfer Universitat Wien Off. of Vector-Borne Disease Control Mahidol University Dept. Specific Prophylaxis/Trop. M. Medicine The acquisition of resistance to chloroquine by Plasmodium vivax in parts of the Australasian and Malaysian epidemiological zones and hitherto unconfirmed reports of such resistance in neighbouring zones show the need for monitoring the drug response of P. vivax. In this study, a recently developed in-vitro microtechnique for the assessment of the parasite's sensitivity to chloroquine was adapted to and validated for lumefantrine. In 21 P. vivax isolates tested at Mae Hong Son, northwestern Thailand, in 2001, the mean geometric cut-off concentration for full inhibition for lumefantrine was 2080 nM. The EC50 and EC90 were 12 nM and 237 nM, respectively. The response was well within the putative therapeutic range. Sensitivity to chloroquine was assessed in 18 P. vivax isolates, showing a geometric mean cut-off concentration of 1095 nM and a wider variation of the individual cut-off points compared with lumefantrine. The EC50 and EC 90 for chloroquine were 16 nM and 511 nM, respectively. One of the isolates, from Myanmar, showed low sensitivity to chloroquine. 2018-07-24T03:28:22Z 2018-07-24T03:28:22Z 2003-12-01 Conference Paper Wiener Klinische Wochenschrift, Supplement. Vol.115, No.3 (2003), 50-54 03005178 2-s2.0-0842291591 https://repository.li.mahidol.ac.th/handle/123456789/21002 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0842291591&origin=inward |
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Medicine Daniela Lux Somsak Prajakwong Herwig Kollaritsch Gunther Wernsdorfer Walther H. Wernsdorfer In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand |
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The acquisition of resistance to chloroquine by Plasmodium vivax in parts of the Australasian and Malaysian epidemiological zones and hitherto unconfirmed reports of such resistance in neighbouring zones show the need for monitoring the drug response of P. vivax. In this study, a recently developed in-vitro microtechnique for the assessment of the parasite's sensitivity to chloroquine was adapted to and validated for lumefantrine. In 21 P. vivax isolates tested at Mae Hong Son, northwestern Thailand, in 2001, the mean geometric cut-off concentration for full inhibition for lumefantrine was 2080 nM. The EC50 and EC90 were 12 nM and 237 nM, respectively. The response was well within the putative therapeutic range. Sensitivity to chloroquine was assessed in 18 P. vivax isolates, showing a geometric mean cut-off concentration of 1095 nM and a wider variation of the individual cut-off points compared with lumefantrine. The EC50 and EC 90 for chloroquine were 16 nM and 511 nM, respectively. One of the isolates, from Myanmar, showed low sensitivity to chloroquine. |
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Universitat Wien |
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Universitat Wien Daniela Lux Somsak Prajakwong Herwig Kollaritsch Gunther Wernsdorfer Walther H. Wernsdorfer |
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Conference or Workshop Item |
author |
Daniela Lux Somsak Prajakwong Herwig Kollaritsch Gunther Wernsdorfer Walther H. Wernsdorfer |
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Daniela Lux |
title |
In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand |
title_short |
In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand |
title_full |
In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand |
title_fullStr |
In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand |
title_full_unstemmed |
In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand |
title_sort |
in-vitro sensitivity testing of plasmodium vivax: response to lumefantrine and chloroquine in northwestern thailand |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/21002 |
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1763490312879079424 |