Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum

To determine if nitric oxide (NO) plays a role in corpus luteum (CL) physiology by affecting progesterone secretion or luteal apoptosis, an in-vitro pseudopregnant rabbit ovarian perfusion system was used to measure the effects of an inhibitor of NO synthesis, NG-nitro-L-arginine methyl ester (L-NAM...

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Main Authors: S. Sangchai Preutthipan, S. H. Chen, J. L. Tilly, K. Kugu, R. R. Lareu, A. M. Dharmarajan
Other Authors: The Johns Hopkins School of Medicine
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/21250
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spelling th-mahidol.212502018-07-24T10:56:02Z Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum S. Sangchai Preutthipan S. H. Chen J. L. Tilly K. Kugu R. R. Lareu A. M. Dharmarajan The Johns Hopkins School of Medicine Mahidol University Saint Barnabas Medical Center Massachusetts General Hospital Graduate School of Medicine and Faculty of Medicine, The University of Tokyo University of Western Australia Biochemistry, Genetics and Molecular Biology Medicine To determine if nitric oxide (NO) plays a role in corpus luteum (CL) physiology by affecting progesterone secretion or luteal apoptosis, an in-vitro pseudopregnant rabbit ovarian perfusion system was used to measure the effects of an inhibitor of NO synthesis, NG-nitro-L-arginine methyl ester (L-NAME), on progesterone secretion and corpus luteal apoptosis as measured by internucleosomal DNA breakdown. Pseudopregnant rabbit ovaries perfused in vitro with L-NAME did not demonstrate any significant differences compared with control ovaries in progesterone secretion. However, apoptosis, as measured by internucleosomal breakdown, was significantly increased in L-NAME-perfused CL compared with controls. While NO does not appear to directly affect progesterone secretion, there does appear to be a role for NO in CL maintenance, or a role for inhibition of NO production in CL regression. 2018-07-24T03:39:06Z 2018-07-24T03:39:06Z 2004-01-01 Article Reproductive BioMedicine Online. Vol.9, No.3 (2004), 264-270 10.1016/S1472-6483(10)62140-2 14726483 2-s2.0-4544269261 https://repository.li.mahidol.ac.th/handle/123456789/21250 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=4544269261&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
S. Sangchai Preutthipan
S. H. Chen
J. L. Tilly
K. Kugu
R. R. Lareu
A. M. Dharmarajan
Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
description To determine if nitric oxide (NO) plays a role in corpus luteum (CL) physiology by affecting progesterone secretion or luteal apoptosis, an in-vitro pseudopregnant rabbit ovarian perfusion system was used to measure the effects of an inhibitor of NO synthesis, NG-nitro-L-arginine methyl ester (L-NAME), on progesterone secretion and corpus luteal apoptosis as measured by internucleosomal DNA breakdown. Pseudopregnant rabbit ovaries perfused in vitro with L-NAME did not demonstrate any significant differences compared with control ovaries in progesterone secretion. However, apoptosis, as measured by internucleosomal breakdown, was significantly increased in L-NAME-perfused CL compared with controls. While NO does not appear to directly affect progesterone secretion, there does appear to be a role for NO in CL maintenance, or a role for inhibition of NO production in CL regression.
author2 The Johns Hopkins School of Medicine
author_facet The Johns Hopkins School of Medicine
S. Sangchai Preutthipan
S. H. Chen
J. L. Tilly
K. Kugu
R. R. Lareu
A. M. Dharmarajan
format Article
author S. Sangchai Preutthipan
S. H. Chen
J. L. Tilly
K. Kugu
R. R. Lareu
A. M. Dharmarajan
author_sort S. Sangchai Preutthipan
title Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
title_short Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
title_full Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
title_fullStr Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
title_full_unstemmed Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
title_sort inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/21250
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